Ke Da-Zhi, Chen Qing-Wei, Li Chun-Li, Li Gui-Qiong
Department of Geriatrics, The Second Affiliated Hospital of Chongqing University of Medical Science, Chongqing 400010, China.
Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(21):2273-7.
To investigate the effect of shenfu injection on canine with cardiogenic shock and the possible mechanism.
Cardiogenic shock model of canine was established by ligating left anterior descending (LAD) of coronary artery. The 15 canines with cardiogenic shock were randomly divided in to glucose injection group, shenfu injection group and sham-operated group. The hemodynamics parameters were monitored. Plasma TNF-alpha and IL-1beta levels were measured by radioimmunoassay. Expression of TNF-alpha mRNA and IL-1beta mRNA in myocardium were detected by RT-PCR.
Following cardiogenic shock, the mean artery pressure (MAP), left ventricular systolic pressure (LVSP), ventricular pressure rise ratio during systolic period (+ dp/dt(max)), and ventricular pressure decay ratio during diastolic period (- dp/dt(max)) decreased significantly; the plasma TNF-alpha and IL-1beta levels and the expression of TNF-a mRNA and IL-1beta mRNA in myocardium increased significantly. In shenfu injection group, MAP, LVSP and +/- dp/dt(max) increased significantly and plasma TNF-alpha and IL-1beta levels decreased significantly. In glucose injection group, MAP, LVSP, +/- dp/dt(max) and plasma TNF-alpha and IL-1beta levels had not changed significantly. The expression of TNF-alpha mRNA and IL-1beta mRNA in myocardium were significantly lower in shenfu injection group than those in glucose injection group.
Shenfu injection probably can decrease over-exprssion of TNF-alpha mRNA and IL-1beta mRNA on transcription platform. Shenfu injection counteract cardiogenic shock, relieve myocardium damage and improve hemodynamics through inhibiting overproduction of TNF-alpha and IL-1beta.
探讨参附注射液对犬心源性休克的影响及可能机制。
通过结扎冠状动脉左前降支建立犬心源性休克模型。将15只心源性休克犬随机分为葡萄糖注射液组、参附注射液组和假手术组。监测血流动力学参数。采用放射免疫分析法测定血浆肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平。通过逆转录聚合酶链反应（RT-PCR）检测心肌中TNF-α mRNA和IL-1β mRNA的表达。
心源性休克后，平均动脉压（MAP）、左心室收缩压（LVSP）、收缩期心室压力上升速率（+dp/dt(max)）和舒张期心室压力下降速率（-dp/dt(max)）显著降低；血浆TNF-α和IL-1β水平以及心肌中TNF-α mRNA和IL-1β mRNA的表达显著增加。参附注射液组中，MAP、LVSP和±dp/dt(max)显著升高，血浆TNF-α和IL-1β水平显著降低。葡萄糖注射液组中，MAP、LVSP、±dp/dt(max)以及血浆TNF-α和IL-1β水平无显著变化。参附注射液组心肌中TNF-α mRNA和IL-1β mRNA的表达显著低于葡萄糖注射液组。
参附注射液可能在转录平台上降低TNF-α mRNA和IL-1β mRNA的过度表达。参附注射液通过抑制TNF-α和IL-1β的过度产生来对抗心源性休克、减轻心肌损伤并改善血流动力学。
