麻风病中红细胞超氧化物歧化酶、过氧化氢酶活性及过氧化氢诱导的脂质过氧化作用

Prasad C V Balasubrahmanya, Kodliwadmath Mallikarjun V, Kodliwadmath Girija Basavaraj

K.L.E.'s Jawaharlal Nehru Medical College, Belgaum 590 010, India.

Lepr Rev. 2007 Dec;78(4):391-7.

OBJECTIVES

To assess erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities and hydrogen peroxide induced lipid peroxidation in leprosy.

DESIGN

One hundred leprosy patients and 50 normal healthy controls were studied for the parameters. The data was analysed by grouping the patients into Ridley-Jopling (RJ) types [Tuberculoid leprosy (TT, n = 22), Borderline tuberculoid leprosy (BT, n = 28), Borderline leprosy (BB, n = 13), Borderline lepromatous leprosy (BL, n = 16) and Lepromatous leprosy (LL, n = 21)] and into different levels of Bacteriological Index (BI) [bacteriologically negative (n = 32), BI = 0.1-1 (n = 22), BI = 1.1-2 (n = 16), BI = 2.1-3 (n = 14), BI = 3.1-4 (n = 10) and BI = 4.1-6 (n = 06)].

RESULTS

The induced peroxidation was significantly high and the enzyme activities were significantly low in leprosy (total patients) as compared to controls. A progressive increase in peroxidation was detected along the leprosy spectrum from TT to LL and the increase was significant in BB, BL and LL groups as compared to controls. Induced peroxidation in LL group as compared to TT, BT and BB and in the BL group as compared to TT and BT were significantly different. A concomitant progressive decline in enzyme activity was detected along the leprosy spectrum from TT to LL. The SOD activity in BB, BL and LL and the CAT activity in BL and LL were significantly low as compared to controls. SOD activity in BB, BL and LL groups as compared to TT and in the LL group as compared to BT were significantly different. A progressive trend of increasing peroxidation and decreasing SOD and CAT activity were also detected along the leprosy groups with advancing level of BI. Induced peroxidation and SOD activity were significantly different in bacteriologically positive groups as compared to controls and in the BI levels 1.1-2, 2.1-3, 3.1-4 and 4.1-6 as compared to bacteriologically negative group. The peroxidation was significantly different in BI levels 2.1-3, 3.1-4 and 4.1-6 as compared to BI level 0.1-1. The CAT activity was significantly different in BI levels 2.1-3, 3.1-4 and 4.1-6 as compared to controls.

CONCLUSION

The study findings suggest oxidative stressful state associated with reduced antioxidant defence potential in erythrocytes of leprosy patients. The study implicates association of erythrocyte oxidative stress with bacterial load and type of leprosy.

目的

评估麻风病患者红细胞超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及过氧化氢诱导的脂质过氧化情况。

设计

对100例麻风病患者和50名正常健康对照者进行这些参数的研究。通过将患者分为里德利 - 乔普林（RJ）类型[结核样型麻风（TT，n = 22）、界线类偏结核样型麻风（BT，n = 28）、界线类麻风（BB，n = 13）、界线类偏瘤型麻风（BL，n = 16）和瘤型麻风（LL，n = 21）]以及不同细菌学指数（BI）水平[细菌学阴性（n = 32）、BI = 0.1 - 1（n = 22）、BI = 1.1 - 2（n = 16）、BI = 2.1 - 3（n = 14）、BI = 3.1 - 4（n = 10）和BI = 4.1 - 6（n = 06）]来分析数据。

结果

与对照组相比，麻风病患者（所有患者）诱导的过氧化显著升高，酶活性显著降低。从TT到LL沿着麻风病光谱检测到过氧化逐渐增加，与对照组相比，BB、BL和LL组的增加显著。LL组与TT、BT和BB组相比以及BL组与TT和BT组相比，诱导的过氧化有显著差异。从TT到LL沿着麻风病光谱检测到酶活性随之逐渐下降。与对照组相比，BB、BL和LL组的SOD活性以及BL和LL组的CAT活性显著降低。BB、BL和LL组与TT组相比以及LL组与BT组相比，SOD活性有显著差异。随着BI水平升高，在麻风病组中也检测到过氧化增加以及SOD和CAT活性降低的渐进趋势。与对照组相比，细菌学阳性组的诱导过氧化和SOD活性有显著差异，与细菌学阴性组相比，BI水平为1.1 - 2、2.1 - 3、3.1 - 4和4.1 - 6时也有显著差异。与BI水平0.1 - 1相比，BI水平2.1 - 3、3.1 - 4和4.1 - 6时的过氧化有显著差异。与对照组相比，BI水平2.1 - 3、3.1 - 4和4.1 - 6时的CAT活性有显著差异。