Dietz Gunnar P H, Bähr Mathias
Neurologische Universitätsklinik, Göttingen, Germany.
Methods Mol Biol. 2007;399:181-98. doi: 10.1007/978-1-59745-504-6_13.
Short basic amino acid sequences, often called cell-penetrating peptides (CPPs), allow the delivery of proteins and other molecules into cells and across the blood-brain barrier (BBB). Although the ability of basic proteins to facilitate such trafficking is known for a long time, only the application of genetic methods and overexpression of fusion proteins in Escherichia coli has lead to a wide application of CPP in many research areas, including signal transduction, cancer, angiogenesis, apoptosis, bone development, cardioprotection, cell cycle, neurobiology, and many others. For the neuroscientist, CPPs are particularly attractive, as a number of articles in the last 5 years have reported their use for neuronal rescue in a number of models for neurodegenerative diseases in vitro and in vivo in rats, mice, or gerbils. Here, we give a detailed description of the protein purification methodology and applications in neuroscience.
短的碱性氨基酸序列，通常称为细胞穿透肽（CPPs），可将蛋白质和其他分子递送至细胞内并穿过血脑屏障（BBB）。尽管碱性蛋白质促进这种运输的能力早已为人所知，但只有通过遗传方法以及在大肠杆菌中融合蛋白的过表达，才使得CPP在许多研究领域得到广泛应用，包括信号转导、癌症、血管生成、细胞凋亡、骨骼发育、心脏保护、细胞周期、神经生物学等等。对于神经科学家而言，CPPs特别具有吸引力，因为在过去5年中有许多文章报道了它们在大鼠、小鼠或沙鼠的多种体外和体内神经退行性疾病模型中用于神经元拯救的情况。在此，我们详细描述了蛋白质纯化方法及其在神经科学中的应用。
