Kato Sayuri E M, Moussatche Nissin, D'Costa Susan M, Bainbridge Travis W, Prins Cindy, Strahl Audra L, Shatzer Amber N, Brinker Alyson J, Kay Nicole E, Condit Richard C
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL, USA.
Virology. 2008 May 25;375(1):213-22. doi: 10.1016/j.virol.2008.01.027. Epub 2008 Mar 7.
Complementation analysis of the combined Condit/Dales collection of vaccinia virus temperature-sensitive mutants has been reported (Lackner, C.A., D'Costa, S.M., Buck, C., Condit, R.C., 2003. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305, 240-259), however not all complementation groups have previously been assigned to single genes on the viral genome. We have used marker rescue to map at least one representative of each complementation group to a unique viral gene. The final combined collection contains 124 temperature-sensitive mutants affecting 38 viral genes, plus five double mutants.
痘苗病毒温度敏感突变体的Condit/Dales联合文库的互补分析已有报道（Lackner, C.A., D'Costa, S.M., Buck, C., Condit, R.C., 2003.痘苗病毒温度敏感突变体的Dales文库的互补分析。病毒学305, 240 - 259），然而之前并非所有互补群都已被定位到病毒基因组上的单个基因。我们利用标记拯救将每个互补群的至少一个代表定位到一个独特的病毒基因上。最终的联合文库包含124个影响38个病毒基因的温度敏感突变体，外加5个双突变体。
