Martínez-Villaluenga Cristina, Frías Juana, Gulewicz Piotr, Gulewicz Krzysztof, Vidal-Valverde Concepción
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
Food Chem Toxicol. 2008 May;46(5):1635-44. doi: 10.1016/j.fct.2008.01.004. Epub 2008 Jan 8.
Three cultivars of broccoli seeds (Brassica oleracea var. italica), cv. Tiburon, cv. Belstar and cv. Lucky, and two cultivars of radish seeds (Raphanus sativus), cv. Rebel and cv. Bolide, were germinated for three and five days and safety aspects such as microbiological counts and biogenic amines were investigated. Cytotoxicity evaluation was also carried out. Broccoli and radish sprouts contained numbers of mesophilic, psychrotrophic, total and faecal coliform bacteria which are the usual counts for minimally processed germinated seeds. Putrescine, cadaverine, histamine, tyramine, spermidine and spermine increased during sprout production although these levels were below those permitted by legislation (5 mg/100 g of edible food). Broccoli and radish sprouts demonstrated no toxic effects on proliferation and viability of HL-60 cells and should be included in our diets as healthy and safe fresh foods.
对三种西兰花种子品种（意大利芥蓝，品种为蒂伯龙、贝尔斯塔和幸运）以及两种萝卜种子品种（萝卜，品种为叛逆者和大力士）进行了3天和5天的发芽处理，并对微生物计数和生物胺等安全方面进行了研究。还进行了细胞毒性评估。西兰花和萝卜芽中含有嗜温菌、嗜冷菌、总大肠菌群和粪大肠菌群数量，这些是最少加工发芽种子的常规计数。在芽苗生产过程中，腐胺、尸胺、组胺、酪胺、亚精胺和精胺有所增加，尽管这些水平低于法规允许的水平（每100克可食用食品5毫克）。西兰花和萝卜芽对HL - 60细胞的增殖和活力没有毒性作用，应作为健康安全的新鲜食品纳入我们的饮食中。
