Dubey J P, Sundar N, Nolden C A, Samuel M D, Velmurugan G V, Bandini L A, Kwok O C H, Bodenstein B, Su C
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705, USA.
J Parasitol. 2007 Dec;93(6):1524-7. doi: 10.1645/GE-1245.1.
During 2005-2006, sera and tissues from raccoons (Procyon lotor), coyotes (Canis latrans), and skunks (Mephitis mephitis) from the state of Wisconsin were tested for Toxoplasma gondii infection. Antibodies to T. gondii were found in 32 of 54 (59.2%) raccoons, 18 of 35 (51.4%) coyotes, and 5 of 7 (71.4%) skunks using the modified agglutination test and a cut-off titer of 1:20. Pooled tissues (brains, hearts, and tongues) from 30 raccoons, 15 coyotes, and 1 skunk were bioassayed for T. gondii infection in mice or cats. Viable T. gondii was isolated from 5 of 30 (16.7%) raccoons, 6 of 15 (40.0%) coyotes, and the skunk. Genetic characterization of the 12 parasite isolates by multilocus PCR-RFLP markers revealed 6 different genotypes including 5 atypical and I archetypal II lineages. The results indicate the prevalence of T. gondii in wildlife mammals is high and that these animals may serve as an important reservoir for transmission of T. gondii.
在2005 - 2006年期间，对来自威斯康星州的浣熊（北美浣熊）、郊狼（犬属）和臭鼬（臭鼬属）的血清和组织进行了弓形虫感染检测。使用改良凝集试验和1:20的截断滴度，在54只浣熊中的32只（59.2%）、35只郊狼中的18只（51.4%）以及7只臭鼬中的5只（71.4%）检测到了抗弓形虫抗体。对30只浣熊、15只郊狼和1只臭鼬的合并组织（脑、心脏和舌头）进行了小鼠或猫的弓形虫感染生物测定。在30只浣熊中的5只（16.7%）、15只郊狼中的6只（40.0%）以及那只臭鼬中分离出了活的弓形虫。通过多位点PCR - RFLP标记对12株寄生虫分离株进行基因特征分析，发现了6种不同的基因型，包括5种非典型和1种原型II型谱系。结果表明野生动物哺乳动物中弓形虫的患病率很高，并且这些动物可能是弓形虫传播的重要宿主。
