Pauchet Yannick, Muck Alexander, Svatos Ales, Heckel David G, Preiss Susanne
Department of Entomology and Mass Spectrometry Research Group, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745 Jena, Germany.
J Proteome Res. 2008 Apr;7(4):1629-39. doi: 10.1021/pr7006208. Epub 2008 Mar 4.
The gut lumen is the primary site of digestion and detoxification and thus presents conditions hostile to most proteins. We used 2D-gel electrophoresis and MS/MS de novo peptide sequencing to identify the major proteins stable enough to persist in the midgut lumen of caterpillars of the cotton bollworm Helicoverpa armigera, a generalist herbivorous insect and a major crop pest worldwide. As expected, we found several enzymes responsible for digestion of carbohydrates, proteins, and lipids. In addition, we identified nondigestive proteins such as a multidomain lipocalin, a protein with pathogen recognition domains, an arginine kinase related to a class of major human allergens, and abundant proteins of unknown function. Identification of the set of proteins that are secreted into the lumen will enable us to further characterize the nutritional and defensive functions of this important intraorganismal space.
肠道腔是消化和解毒的主要场所，因此呈现出对大多数蛋白质不利的环境。我们利用二维凝胶电泳和串联质谱从头肽测序，来鉴定足够稳定从而能在棉铃虫（一种多食性植食性昆虫，也是全球主要的农作物害虫）幼虫中肠腔中持续存在的主要蛋白质。不出所料，我们发现了几种负责碳水化合物、蛋白质和脂质消化的酶。此外，我们还鉴定出了非消化性蛋白质，如一种多结构域脂质运载蛋白、一种具有病原体识别结构域的蛋白质、一种与一类主要人类过敏原相关的精氨酸激酶，以及大量功能未知的蛋白质。鉴定分泌到肠腔中的蛋白质组，将使我们能够进一步表征这个重要的体内空间的营养和防御功能。
