Bullock J, Khan M Y, Ianzano J A
Neoplasma. 1976;23(3):277-83.
Transplantable hepatomas have a special importance in cancer research because most are well-differentiated and grow at widely differing rates. A simple and reproducible technique was developed to transplant the rapidly growing Morris hepatoma 9618A2 from a subcutaneous to an intrahepatic site. The tumor has been maintained for over 80 successive transplant generations by inoculating Buffalo rats subcutaneously. The hepatoma, transplanted many times from the subcutaneous site to the liver, was examined histologically on various occasions. By varying inoculum size, growth of the hepatocarcinoma could be controlled. Throughout transplantation, the donor animals were bled without causing the death of the animal. Histological studies revealed that the tumor, when transplanted intrahepatically, remains a highly differentiated neoplasm with no observable morphological changes.
可移植性肝癌在癌症研究中具有特殊的重要性，因为大多数肝癌分化良好，且生长速度差异很大。我们开发了一种简单且可重复的技术，将快速生长的莫里斯肝癌9618A2从皮下移植到肝内。通过皮下接种布法罗大鼠，该肿瘤已连续维持了80多个移植代。多次从皮下部位移植到肝脏的肝癌，在不同时间进行了组织学检查。通过改变接种量，可以控制肝癌的生长。在整个移植过程中，供体动物被采血而不导致动物死亡。组织学研究表明，该肿瘤在肝内移植时，仍然是高度分化的肿瘤，没有可观察到的形态学变化。
