Lin Jun-Jie, Xu Zhong, Chen Ming, Qiu Ying-Jie, Zhang Xi, Kong Xiang-Rong, Zhou Xiao-Yan, Ma Qing, Qian Kai-Chen
Shanghai Blood Center, Shanghai 200051, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Feb;16(1):189-91.
This study was purposed to investigate the effectiveness of bacterial screening with 24 hours holding in preventing and controlling bacterial contamination of platelets. Bacterial screening of apheresis platelets preserved for 24 hours was performed by using BacT/ALERT automatic bacterial culture system. The samples from 5 bags of platelet were taken in aseptic condition and were merged into 1 bag. The final sample was inoculated into aerobic and anaerobic bottle respectively for testing, meanwhile the screened platelet samples were held for 24 hours. If the platelets were cultured for 24 hours and identification of bacterial strains showed negative, the platelets could be released, and the original platelet samples should be rescreened if initiate positive was found. The results showed that in screening 8017 samples of apheresis platelets the initiate positive results were 16 (0.2%) and confirmed positive were 4 (0.05%). Out of 4 confirmed positive strains, three were Staphylococcus aureus and another was Staphylococcus auricularis. It is concluded that it is necessary for blood center to apply the method of bacterial screening of platelet with 24 hours holding as conventional screening method, which is an effective and feasible way to prevent and control bacterial contamination of platelets.
本研究旨在探讨血小板保存24小时后进行细菌筛查在预防和控制血小板细菌污染方面的有效性。采用BacT/ALERT自动细菌培养系统对单采血小板保存24小时后进行细菌筛查。在无菌条件下从5袋血小板中取样并合并为1袋。将最终样本分别接种到需氧瓶和厌氧瓶中进行检测，同时将筛查的血小板样本保存24小时。若血小板培养24小时且细菌菌株鉴定为阴性，则血小板可放行，若初筛阳性则应对原始血小板样本重新进行筛查。结果显示，在筛查8017份单采血小板样本中，初筛阳性结果为16份（0.2%），确认阳性为4份（0.05%）。在4株确认阳性菌株中，3株为金黄色葡萄球菌，另1株为耳葡萄球菌。结论是，血站有必要将血小板保存24小时后的细菌筛查方法作为常规筛查方法应用，这是预防和控制血小板细菌污染的有效可行方法。
