Joshi S, Huang Y G
Department of Cell and Molecular Biology, Boston Biomedical Research institute 02114.
Biochim Biophys Acta. 1991 Aug 26;1067(2):255-8. doi: 10.1016/0005-2736(91)90051-9.
The requirement of bovine heart mitochondrial oligomycin sensitivity conferring protein (OSCP) in conferring dicyclohexylcarbodiimide (DCCD)-sensitivity to membrane-bound F1 was investigated by using OSCP-depleted membrane fraction (UF0) of ATP synthase. The ATPase activity of UF0-F1 was completely insensitive to DCCD while that of UF0-F1-OSCP was inhibited 95% by 16 microM DCCD. Both UF0-F1 and UF0-F1-OSCP complexes bound 5 nmol [14C]DCCD/mg UF0, and all the radioactivity was found to be associated with the DCCD-binding proteolipid. The data suggest that OSCP may be necessary for transmitting not only energy-linked signals, but also signals induced by F0 inhibitory ligands in mitochondrial energy transduction.
利用ATP合酶的寡霉素敏感性赋予蛋白（OSCP）缺失的膜组分（UF0），研究了牛心线粒体寡霉素敏感性赋予蛋白（OSCP）在赋予膜结合F1对二环己基碳二亚胺（DCCD）敏感性方面的作用。UF0 - F1的ATP酶活性对DCCD完全不敏感，而UF0 - F1 - OSCP的ATP酶活性在16 μM DCCD作用下被抑制了95%。UF0 - F1和UF0 - F1 - OSCP复合物均结合5 nmol [14C]DCCD/mg UF0，并且所有放射性均与DCCD结合的蛋白脂质相关。数据表明，OSCP不仅对于传递能量相关信号可能是必需的，而且对于传递线粒体能量转导中由F0抑制性配体诱导的信号可能也是必需的。
