Kahng Jimin, Park Hun Hee, Han Kyungja, Choi Bo Yeon, Lee Wonbae
Department of Clinical Pathology, College of Medicine, Catholic University of Korea, Seoul, Korea.
Ann Clin Lab Sci. 2008 Winter;38(1):6-11.
The numbers of antibody-binding sites of platelet glycoprotein (GP) IIb/IIIa on circulating platelets were analyzed using 4 kinds of antibodies in 34 aplastic anemia (AA) patients, 20 idiopathic thrombocytopenic purpura (ITP) patients, and 14 normal controls. The numbers of antibody-binding sites of CD41, CD41a, CD41b, and CD61 on platelets of the AA patients were less than in the normal controls (p <0.001). In the ITP patients, the numbers of sites for CD41 and CD41a were less than in normal controls (p <0.05). There were significant positive correlations between CD41 and CD41a, CD41b, and CD61 in the 3 groups. There were significant negative correlations between CD41 and CD41b and between CD41a and CD41b in the normal controls, but not in the AA or ITP patients. In summary, the numbers of the 4 antibody-binding sites of GPIIb/IIIa on platelets of AA and ITP patients are different from those in normal controls. Measurements of the antibody-binding sites of GPIIb/IIIa are not necessary for the differential diagnosis of AA and ITP. However, the differences in correlations between the numbers of epitopes in AA and ITP patients suggest that the epitopes of GPIIb/IIIa are altered in these diseases.
使用4种抗体对34例再生障碍性贫血（AA）患者、20例特发性血小板减少性紫癜（ITP）患者和14名正常对照者循环血小板上血小板糖蛋白（GP）IIb/IIIa的抗体结合位点数量进行了分析。AA患者血小板上CD41、CD41a、CD41b和CD61的抗体结合位点数量少于正常对照者（p<0.001）。ITP患者中，CD41和CD41a的位点数量少于正常对照者（p<0.05）。3组中CD41与CD41a、CD41b和CD61之间存在显著正相关。正常对照者中CD41与CD41b之间以及CD41a与CD41b之间存在显著负相关，但AA或ITP患者中不存在。总之，AA和ITP患者血小板上GPIIb/IIIa的4个抗体结合位点数量与正常对照者不同。测量GPIIb/IIIa的抗体结合位点对AA和ITP的鉴别诊断并非必要。然而，AA和ITP患者中表位数量之间相关性的差异表明，这些疾病中GPIIb/IIIa的表位发生了改变。
