Poutsiaka D D, Clark B D, Vannier E, Dinarello C A
Department of Medicine, New England Medical Center Hospitals, Boston, MA.
Blood. 1991 Sep 1;78(5):1275-81.
We studied the relationship between the production of the 23-Kd interleukin-1 receptor antagonist (IL-1ra) and IL-1 beta in cultures of human peripheral blood mononuclear cells (PBMC) using a specific radioimmunoassay for IL-1ra that had a sensitivity of 166 +/- 11 pg/mL. PBMC cultured without human serum made little IL-1ra or IL-1 beta. In the presence of 1% AB serum, there was no increase in IL-1 beta (0.25 +/- 0.13 ng/mL) but IL-1ra production increased sevenfold to 3.4 +/- 0.5 ng/mL. IgG (2.5 to 100 micrograms/mL IgG) or granulocyte-macrophage colony-stimulating factor (GM-CSF) (1 to 100 ng/mL) had no significant effect on IL-1 beta production but increased IL-1ra production up to 18-fold (18.2 +/- 3.9 ng/mL). Using endotoxin as a stimulant, 82% +/- 2% of IL-1ra was secreted in comparison with 52% +/- 9% of IL-1 beta. Culture conditions of PBMC influenced the production of IL-1ra but not IL-1 beta. Rocking endotoxin-stimulated PBMC produced 75% less IL-1ra but the same amount of IL-1 beta when compared with PBMC cultured in stationary plastic tubes. Rocking IgG-or GM-CSF-stimulated PBMC also produced 75% to 80% less IL-1ra. GM-CSF or IL-1 beta at concentrations that elicited submaximal production of IL-1ra potentiated IgG-induced IL-1ra production. The production of IL-1ra and IL-1 beta are under differential regulation because serum, IgG, and GM-CSF were potent stimuli for the production of IL-1ra but not IL-1 beta, and the prevention of cell-cell contact of PBMC reduced IL-1ra but not IL-1 beta production.
我们使用一种对白细胞介素 -1受体拮抗剂（IL -1ra）灵敏度为166±11 pg/mL的特异性放射免疫分析法，研究了人外周血单个核细胞（PBMC）培养物中23 -Kd的IL -1ra产生与IL -1β之间的关系。在无人类血清的情况下培养的PBMC几乎不产生IL -1ra或IL -1β。在存在1% AB血清的情况下，IL -1β没有增加（0.25±0.13 ng/mL），但IL -1ra的产生增加了7倍，达到3.4±0.5 ng/mL。免疫球蛋白G（IgG，2.5至100微克/毫升IgG）或粒细胞 - 巨噬细胞集落刺激因子（GM -CSF，1至100纳克/毫升）对IL -1β的产生没有显著影响，但可使IL -1ra的产生增加至18倍（18.2±3.9 ng/mL）。以内毒素作为刺激物时，82%±2%的IL -1ra被分泌，而IL -1β的分泌比例为52%±9%。PBMC的培养条件影响IL -1ra的产生，但不影响IL -1β的产生。与在固定塑料管中培养的PBMC相比，摇晃内毒素刺激的PBMC产生的IL -1ra减少75%，但IL -1β的产生量相同。摇晃IgG或GM -CSF刺激的PBMC产生的IL -1ra也减少75%至80%。在引起IL -1ra产生未达最大值的浓度下，GM -CSF或IL -1β可增强IgG诱导的IL -1ra产生。IL -1ra和IL -1β的产生受到不同的调节，因为血清、IgG和GM -CSF是IL -1ra产生的有效刺激物，但不是IL -1β产生的有效刺激物，并且防止PBMC的细胞间接触会减少IL -1ra的产生，但不会减少IL -1β的产生。
