矮生豌豆上胚轴中S-腺苷甲硫氨酸合成酶和S-腺苷甲硫氨酸水平的植物激素调控
Phytohormonal regulation of S-adenosylmethionine synthetase and S-adenosylmethionine levels in dwarf pea epicotyls.
作者信息
Mathur M, Sachar R C
机构信息
Department of Botany, University of Delhi, India.
出版信息
FEBS Lett. 1991 Aug 5;287(1-2):113-7. doi: 10.1016/0014-5793(91)80028-2.
A significant stimulation (2- to 2.5-fold) of AdoMet synthetase was witnessed in glibberellicd acid (GA3, 1 microM)-treated epicotyls of the dwarf pea (Pisum sativum). This was accompanied by a 2.4-fold increase in the endogenous pool of S-adenosylmethionine. Both abscisic acid (10 microM) and cycloheximide (20 micrograms/ml) inhibited the GA3-mediated enhancement of AdoMet synthetase activity. Three isozymes of AdoMet synthetase were detected in GA3-treated epicotyls, whereas a single activity peak was observed in controls. Thus, GA3 seems to control the induction of two new isozymes of AdoMet synthetase in the dwarf pea. By contrast, the tall pea exhibited three isozymes of AdoMet synthetase even in the absence of GA3 treatment. High concentration of L-methionine (2 mM) mimicked the GA3-elicited induction of two new isozymes of AdoMet synthetase in dwarf pea epicotyls.
在赤霉素（GA3，1微摩尔）处理的矮豌豆（豌豆）上胚轴中，观察到腺苷甲硫氨酸合成酶有显著刺激（2至2.5倍）。这伴随着S-腺苷甲硫氨酸内源性池增加2.4倍。脱落酸（10微摩尔）和环己酰亚胺（20微克/毫升）均抑制GA3介导的腺苷甲硫氨酸合成酶活性增强。在GA3处理的上胚轴中检测到三种腺苷甲硫氨酸合成酶同工酶，而在对照中观察到一个单一活性峰。因此，GA3似乎控制了矮豌豆中两种新的腺苷甲硫氨酸合成酶同工酶的诱导。相比之下，即使在没有GA3处理的情况下，高豌豆也表现出三种腺苷甲硫氨酸合成酶同工酶。高浓度的L-甲硫氨酸（2毫摩尔）模拟了GA3诱导的矮豌豆上胚轴中两种新的腺苷甲硫氨酸合成酶同工酶的诱导。
Zotero 插件