Choi Seung-Cheol, Choi Ji-Hyun, Shim Wan-Joo, Lim Do-Sun
Department of Cardiology, College of Medicine, Korea University, Seoul, 136-705, Republic of Korea.
Biotechnol Lett. 2008 Jul;30(7):1169-75. doi: 10.1007/s10529-008-9677-6. Epub 2008 Mar 4.
P19 embryonal carcinoma cells were examined under different conditions of embryoid body (EB) formation to evaluate endothelial differentiation. A prominent capillary-like network including PECAM-1 positive cells and upregulated expression of endothelial markers Tie-1, Tie-2, Flk-1 and PECAM-1 were identified in the DMSO-treated group during EB formation as well as after VEGF treatment. About 4% of the P19 cells differentiated into PECAM-1 positive cells on day 14 of differentiation. These results suggest that the P19 cells have the potential for endothelial cell differentiation and provide a new model system for future research.
在不同的胚状体（EB）形成条件下检查P19胚胎癌细胞，以评估内皮细胞分化情况。在EB形成过程中以及VEGF处理后，在二甲基亚砜（DMSO）处理组中鉴定出一个显著的毛细血管样网络，其中包括血小板内皮细胞黏附分子-1（PECAM-1）阳性细胞以及内皮标志物Tie-1、Tie-2、Flk-1和PECAM-1的表达上调。在分化第14天，约4%的P19细胞分化为PECAM-1阳性细胞。这些结果表明，P19细胞具有向内皮细胞分化的潜力，并为未来的研究提供了一个新的模型系统。
