Gwak So-Jung, Kim Byung-Soo
Department of Chemical Engineering, Hanyang University, Seoul, 133-791, Korea.
Biotechnol Lett. 2008 Jul;30(7):1177-82. doi: 10.1007/s10529-008-9676-7. Epub 2008 Mar 4.
Polyethylenimine (PEI) is one of the most extensively studied non-viral vectors but its cytotoxicity limits its clinical value. PLGA nanospheres are biocompatible and can facilitate sustained release of plasmid DNA. This study compares the cytotoxicity and long-term transgene expression between PLGA nanosphere and PEI. PLGA nanospheres were significantly less cytotoxic than PEI at various concentrations. PLGA nanospheres induced significantly higher transgene expression in vitro for a longer duration (21 days) than PEI. We conclude that PLGA nanospheres have potential as gene delivery vehicles for use in gene therapy for diseases in which a long-term therapeutic gene expression regimen is necessary.
聚乙烯亚胺（PEI）是研究最为广泛的非病毒载体之一，但其细胞毒性限制了其临床应用价值。聚乳酸-羟基乙酸共聚物（PLGA）纳米球具有生物相容性，能够促进质粒DNA的持续释放。本研究比较了PLGA纳米球与PEI的细胞毒性及长期转基因表达情况。在不同浓度下，PLGA纳米球的细胞毒性显著低于PEI。PLGA纳米球在体外诱导的转基因表达显著高于PEI，且持续时间更长（21天）。我们得出结论，对于需要长期治疗性基因表达方案的疾病，PLGA纳米球有潜力作为基因治疗的基因递送载体。
