Fowler Larry J, Lachar Whitney A
Department of Pathology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, Mail Code #7750, San Antonio, TX 78229-3900, USA.
Arch Pathol Lab Med. 2008 Mar;132(3):373-83. doi: 10.5858/2008-132-373-AOITC.
The uses of monoclonal antibodies via immunochemistry have been reported frequently within the literature using various methodologies with applications to cytology specimens. The direct application of immunochemistry to cytology may have a variety of pitfalls that the general pathologist familiar with its application to histology may be unaware of when applying it prospectively to patient specimens.
To review common pitfalls when applying immunochemistry to cytology specimens and to suggest approaches to the more common differential dilemmas that apply to a variety of cytology specimens that could be seen in a general pathology practice.
The authors' own experiences of applying immunochemistry to cytopathology specimens within an academic setting along with supportive data from the literature.
Immunochemistry can be used to increase the predictability of a cytology diagnosis if care is taken with the cytology sample preparation methodology and there is judicious use of select monoclonal antibody panels to support a specific cytology diagnosis. Up-to-date evidence-based antibody databases should be used when selecting antibody panels.
文献中经常报道通过免疫化学方法使用单克隆抗体，采用各种方法应用于细胞学标本。免疫化学直接应用于细胞学可能存在各种陷阱，熟悉其在组织学中应用的普通病理学家在将其前瞻性地应用于患者标本时可能并未意识到这些问题。
回顾将免疫化学应用于细胞学标本时的常见陷阱，并针对一般病理实践中可能遇到的各种细胞学标本的更常见鉴别难题提出解决方法。
作者在学术环境中将免疫化学应用于细胞病理学标本的自身经验以及文献中的支持性数据。
如果在细胞学样本制备方法上小心谨慎，并明智地使用选定的单克隆抗体组合来支持特定的细胞学诊断，免疫化学可用于提高细胞学诊断的可预测性。选择抗体组合时应使用最新的循证抗体数据库。
