通过间接免疫荧光法检测抗双链DNA抗体、抗线粒体抗体以及免疫印迹法检测自身抗体与自身免疫性疾病临床管理中相应数据的相关性。
Correlation of autoantibodies presence detected by IFA-anti-dsDNA, IFA-AMA and immunoblotting with corresponding data in clinical management of autoimmune diseases.
作者信息
Subasić Demo, Karamehić Jasenko, Ljuca Farid, Gavrankapetanović Faris, Delić-Sarac Marina, Eminović Izet, Kovacević Dragan
机构信息
Institute of Clinical Immunology - University of Sarajevo Clinics Centre, Bolnicka 25, 71000 Sarajevo, Bosnia and Herzegovina.
出版信息
Bosn J Basic Med Sci. 2008 Feb;8(1):86-92. doi: 10.17305/bjbms.2008.3004.
Diagnosis and management of patients with SLE (Systemic Lupus Eritematosus), autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), involves specific diagnostic tests, such as IFA-AMA, IFA anti-dsDNA and immunoblotting for the detection of autoantibodies for specific autoantigens (mitochondria, dsDNA, M2, LKM-1, LC-1, SLA/LP). We established specific correlation between the detected autoantibodies and corresponding clinical findings. The total of 813 serum specimens were probed with IFA-anti-dsDNA, 98 of which tested positive. We also performed dilution analysis to the end point for all the positive specimens. Numerous specimens were tested by IFA, AMA and immunoblotting.
系统性红斑狼疮（SLE）、自身免疫性肝炎（AIH）、原发性胆汁性肝硬化（PBC）患者的诊断和管理涉及特定的诊断测试，如间接荧光抗体法检测抗线粒体抗体（IFA-AMA）、间接荧光抗体法检测抗双链DNA抗体（IFA anti-dsDNA）以及用于检测针对特定自身抗原（线粒体、双链DNA、M2、肝肾微粒体-1、肝溶质抗原-1、可溶性肝抗原/肝胰抗原）自身抗体的免疫印迹法。我们建立了检测到的自身抗体与相应临床发现之间的特定关联。用间接荧光抗体法检测抗双链DNA对总共813份血清标本进行了检测，其中98份呈阳性。我们还对所有阳性标本进行了终点稀释分析。许多标本通过间接荧光抗体法、抗线粒体抗体检测和免疫印迹法进行了检测。
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