Musarrat J, Ahmad M
Interdisciplinary Biotechnology Unit, Faculty of Life Sciences, Aligarh Muslim University, India.
Mutagenesis. 1991 May;6(3):207-11. doi: 10.1093/mutage/6.3.207.
Bacteriophage lambda-Escherichia coli complexes exhibited remarkable sensitivity to alkaline pH 10.0 at 37 degrees C. The decline in plaque forming units after alkali treatment was more pronounced in complexes with some of the radiation repair defective mutants of E. coli K-12, i.e. uvrArecA, recA, rer and lexA mutants as compared to those of uvrA, recB and wild-type strains. The red gene of lambda phage and recA gene of E. coli seem to have a complementary effect on the alkali induced lesions. Alkaline treatment to lysogenic lambda phage was also found to be mutagenic. An enhanced level of mutagenesis was observed when treated phage particles were allowed to adsorb on treated wild-type bacteria. Moreover, the alkali treatment to lysogen (lambda cI857-E. coli) resulted in prophage induction in nutrient broth even at 32 degrees C. Thus on the basis of these results the role of error prone SOS repair systems in the repair of alkali induced lesions in lysogenic bacteriophage lambda has been suggested.
λ噬菌体 - 大肠杆菌复合物在37℃下对碱性pH 10.0表现出显著的敏感性。与uvrA、recB和野生型菌株相比，用碱处理后，噬菌斑形成单位的下降在与一些大肠杆菌K - 12的辐射修复缺陷突变体（即uvrArecA、recA、rer和lexA突变体）形成的复合物中更为明显。λ噬菌体的red基因和大肠杆菌的recA基因似乎对碱诱导的损伤具有互补作用。还发现对溶原性λ噬菌体进行碱性处理具有诱变作用。当处理过的噬菌体颗粒吸附在处理过的野生型细菌上时，观察到诱变水平增强。此外，对溶原菌（λcI857 - 大肠杆菌）进行碱处理甚至在32℃时也会在营养肉汤中导致原噬菌体诱导。因此，基于这些结果，有人提出了易错SOS修复系统在修复溶原性噬菌体λ中的碱诱导损伤中的作用。
