Luo Li-Hua, Wang Xiao-Mei, Zhang Yu-Feg, Liu Yong-Ming, Chang Peter R, Wang Yan, Chen Yun
Research Center for Medical and Structural Biology, School of Basic Medical Science, Wuhan University, Wuhan, China.
J Biomater Sci Polym Ed. 2008;19(4):479-96. doi: 10.1163/156856208783719491.
A series of cellulose/soy protein isolate (SPI) membranes was prepared from cellulose and SPI solution by casting and coagulation from 5 wt% acetic acid and 5 wt% sulphuric acid aqueous solution, respectively. The structure and properties of the membranes were characterized by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy and tensile testing. The effects of SPI content (W(SPI)) and the coagulants on the structure and properties of the membranes were investigated. The membranes exhibited porous structure. The pore size in the surfaces and cross-sections of the membranes increased with an increase of W(SPI) regardless of the coagulants. The membranes containing 10 wt% W(SPI) showed higher tensile strength and elongation at break than other membranes. The membranes with the same W(SPI) coagulated from acetic acid solution exhibited higher values of tensile strength, elongation at break and pore size in the surfaces and cross-sections than those corresponding membranes coagulated from sulphuric acid. The biocompatibility of the acetic acid-coagulated membranes was preliminarily evaluated by cell culture and in vivo implantation experiments. The results revealed that human umbilical vein endothelial cells (ECV304) grew well on this biomaterial. In comparison with the pure cellulose membrane, because of the incorporation of SPI and the resultant alteration of microstructure, the SPI-modified membranes showed an improved in vivo biocompatibility and biodegradability in the implantation experiments. These cellulose/SPI membranes warrant further explorations in biomedical fields.
通过分别从5 wt%的醋酸水溶液和5 wt%的硫酸水溶液中浇铸和凝固，由纤维素和大豆分离蛋白（SPI）溶液制备了一系列纤维素/大豆分离蛋白（SPI）膜。通过傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和拉伸试验对膜的结构和性能进行了表征。研究了SPI含量（W(SPI)）和凝固剂对膜结构和性能的影响。膜呈现出多孔结构。无论凝固剂如何，膜表面和横截面的孔径都随着W(SPI)的增加而增大。含有10 wt% W(SPI)的膜比其他膜表现出更高的拉伸强度和断裂伸长率。从醋酸溶液中凝固的具有相同W(SPI)的膜在拉伸强度、断裂伸长率以及表面和横截面的孔径方面均高于从硫酸中凝固的相应膜。通过细胞培养和体内植入实验对醋酸凝固膜的生物相容性进行了初步评估。结果表明，人脐静脉内皮细胞（ECV304）在这种生物材料上生长良好。与纯纤维素膜相比，由于SPI的掺入以及由此导致的微观结构变化，SPI改性膜在植入实验中表现出更好的体内生物相容性和生物降解性。这些纤维素/SPI膜在生物医学领域值得进一步探索。
