Zhang Wayne W, Hasaniya Nahidh W, Premaratne Shyamal, McNamara J Judson
Department of Surgery, School of Medicine, Loma Linda University, 11175 Campus Street, Loma Linda, CA 92354, USA.
Vasc Endovascular Surg. 2008 Jun-Jul;42(3):263-7. doi: 10.1177/1538574408314438. Epub 2008 Mar 4.
The aim of this study is to investigate whether atrial natriuretic peptide can mimic preconditioning to protect ischemia or reperfusion injury in rabbit hearts. New Zealand white rabbits were randomized into 3 groups: (1) Controls. Hearts received a 60 minute-occlusion of the left anterior descending artery, followed by a 180 minute-reperfusion. (2) Preconditioning. Two 5-minute periods of ischemia separated by a 10-minute reperfusion, followed by a 60-minute ischemia and a 180-minute reperfusion. (3) Atrial natriuretic peptide treatment. Bolus injection of exogenous atrial natriuretic peptide (2.5 microg/kg) given intravenously at 15 minutes prior to 60 minute-ischemia followed by a 180-minute reperfusion. Myocardial necrotic area and area at risk of necrosis were determined by triphenyltetrazolium chloride staining. Ratio of necrotic area to area at risk was 49.95% +/- 1.15%, 7.95% +/- 0.33%, and 8.36% +/- 0.61% in the controls, preconditioning group, and atrial natriuretic peptide group, respectively. Both preconditioning and atrial natriuretic peptide significantly reduced the size of infarct caused by ischemia (preconditioning vs controls, P < .05; atrial natriuretic peptide vs controls, P < .05). Atrial natriuretic peptide can mimic ischemic preconditioning to protect rabbit hearts from prolonged ischemia and reperfusion injury. It may be involved in the cardioprotective mechanisms of preconditioning.
本研究的目的是探讨心房利钠肽是否能模拟预处理来保护兔心脏免受缺血或再灌注损伤。将新西兰白兔随机分为3组：（1）对照组。心脏接受左前降支动脉60分钟的闭塞，随后进行180分钟的再灌注。（2）预处理组。两次5分钟的缺血期，中间间隔10分钟的再灌注，随后进行60分钟的缺血和180分钟的再灌注。（3）心房利钠肽治疗组。在60分钟缺血前15分钟静脉推注外源性心房利钠肽（2.5微克/千克），随后进行180分钟的再灌注。通过氯化三苯基四氮唑染色确定心肌坏死面积和坏死危险区面积。对照组、预处理组和心房利钠肽组的坏死面积与坏死危险区面积之比分别为49.95%±1.15%、7.95%±0.33%和8.36%±0.61%。预处理和心房利钠肽均显著减小了缺血所致梗死灶的大小（预处理组与对照组比较，P<0.05；心房利钠肽组与对照组比较，P<0.05）。心房利钠肽可模拟缺血预处理保护兔心脏免受长时间缺血和再灌注损伤。它可能参与了预处理的心脏保护机制。
