Odbileg Raadan, Purevtseren Byambaa, Gantsetseg Dorj, Boldbaatar Bazartseren, Buyannemekh Tumurjav, Galmandakh Zagd, Erdenebaatar Janchivdorj, Konnai Satoru, Onuma Misao, Ohashi Kazuhiko
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
J Vet Med Sci. 2008 Feb;70(2):197-201. doi: 10.1292/jvms.70.197.
In the present study, we determined the levels of cytokines produced by camel (Camelus bactrianus) peripheral blood mononuclear cells (PBMCs) in response to live attenuated Brucella abortus (B. abortus) S19 vaccine. Seven camels were vaccinated with commercial B. abortus S19 vaccine, and their cytokine responses were determined using a real-time PCR assay. Cytokine responses to B. abortus S19 were examined at 6 hr, 48 hr and 1, 2 and 3 weeks post-vaccination. Serological tests were performed to further confirm these immune responses. The results revealed that IFN-gamma and IL-6 were upregulated during the first week post-vaccination. Low level expressions of IL-1alpha, IL-1beta, TNFalpha and IL-10 and no expression of IL-2 and IL-4 were observed compared with the control camels. The findings showed that B. abortus stimulates cell-mediated immunity by directly activating camel Th1 cells to secrete IFN-gamma. This quantification of cytokine expression in camels is essential for understanding of Camelidae disease development and protective immune responses. This is the first report of in vivo camel cytokine quantification after vaccination.
在本研究中，我们测定了双峰驼外周血单核细胞（PBMCs）对减毒活布鲁氏菌（B. abortus）S19疫苗产生的细胞因子水平。七只双峰驼接种了商业化的B. abortus S19疫苗，并使用实时PCR测定法确定它们的细胞因子反应。在接种疫苗后的6小时、48小时以及1、2和3周时检查对B. abortus S19的细胞因子反应。进行血清学检测以进一步确认这些免疫反应。结果显示，接种疫苗后的第一周，IFN-γ和IL-6上调。与对照双峰驼相比，观察到IL-1α、IL-1β、TNFα和IL-10的低水平表达，且未观察到IL-2和IL-4的表达。研究结果表明，B. abortus通过直接激活双峰驼Th1细胞分泌IFN-γ来刺激细胞介导的免疫。双峰驼中细胞因子表达的这种定量对于理解骆驼科疾病的发展和保护性免疫反应至关重要。这是接种疫苗后双峰驼体内细胞因子定量的首次报告。
