杨梅素是否通过钙调蛋白介导对人红细胞钙泵ATP酶的抑制作用？

Does calmodulin mediate inhibition of human erythrocyte Ca(2+)-pumping ATPase by myricetin?

作者信息

Teo T S, Thiyagarajah P, Wong Z Z, Das N P

机构信息

Department of Biochemistry, National University of Singapore, Kent Ridge.

出版信息

Biochem Med Metab Biol. 1991 Apr;45(2):209-15. doi: 10.1016/0885-4505(91)90023-e.

DOI:10.1016/0885-4505(91)90023-e

PMID:1831992

Abstract

Myricetin, a flavonoid, potently inhibits human erythrocyte plasma membrane Ca(2+)-pumping ATPase activity: half-maximal inhibition of the basal and calmodulin-stimulated activity is obtained with 6 microM myricetin. Inhibition of the Ca(2+)-ATPase by myricetin is observed at all concentrations of free Ca2+ from 10(-8)M to 10(-5)M. The extent of inhibition of the Ca(2+)-ATPase is dependent on the time of preincubation of the plasma membranes with myricetin. Inhibition of the Ca(2+)-ATPase by myricetin is not reversed by excess calmodulin. It is concluded that calmodulin does not mediate myricetin's inhibition of human erythrocyte plasma membrane Ca(2+)-pumping ATPase.

摘要

杨梅素是一种黄酮类化合物，能有效抑制人红细胞质膜Ca(2+)-泵ATP酶活性：6微摩尔杨梅素可使基础活性和钙调蛋白刺激的活性受到半数最大抑制。在10(-8)M至10(-5)M的所有游离Ca2+浓度下，均观察到杨梅素对Ca(2+)-ATP酶的抑制作用。Ca(2+)-ATP酶的抑制程度取决于质膜与杨梅素预孵育的时间。过量的钙调蛋白不能逆转杨梅素对Ca(2+)-ATP酶的抑制作用。结论是钙调蛋白不介导杨梅素对人红细胞质膜Ca(2+)-泵ATP酶的抑制作用。