Lenz P
Institut National de la Santé et de la Recherche Médicale, Unité 281, Directeur D Cathignol, 69424 Lyon, France.
Appl Opt. 1999 Jun 1;38(16):3662-9. doi: 10.1364/ao.38.003662.
Samples of different animal tissues containing, at variable depth, a thin fluorescent sheet are irradiated with continuous violet or red light or with nonlinearly absorbed pulsed infrared light. The fluorescence intensity measured at the tissue surface as a function of the location of the fluorescent sheet exhibits, after a transition zone close to the tissue surface, an exponential decrease, the slope of which depends on the optical penetration depths of the exciting and the fluorescent light. From these results the total fluorescence output is determined for specific fluorophor distributions. It is seen that considerably deeper tissue layers are explored by use of excitation with red instead of violet light. Nonlinear excitation by infrared light can provide a further improvement, especially in liver tissue.
含有不同深度薄荧光片的不同动物组织样本，用连续紫光或红光或非线性吸收的脉冲红外光进行照射。在组织表面测量的荧光强度作为荧光片位置的函数，在靠近组织表面的过渡区之后呈现指数下降，其斜率取决于激发光和荧光的光学穿透深度。根据这些结果，确定特定荧光团分布的总荧光输出。可以看出，使用红光而非紫光激发能够探测到更深的组织层。红外光的非线性激发可以进一步改善，尤其是在肝脏组织中。
