Garny Kerstin, Horn Harald, Neu Thomas R
Institute of Water Quality Control, Technische Universität München, Am Coulombwall, Garching, Germany.
Bioprocess Biosyst Eng. 2008 Oct;31(6):619-29. doi: 10.1007/s00449-008-0212-x. Epub 2008 Mar 5.
In biotechnology, composition of biofilms and suspended bioaggregates can be crucial for system performance or product quality. Consequently, understanding biofilm dynamics is important for any process optimisation. The aim of this study was to investigate biofilm development and detachment under different hydrodynamic conditions and varying glucose load. Confocal laser scanning microscopy proved to be a fast method providing information about structure, distribution and volume ratio of bacteria and extra cellular polymers (EPS) within biofilms and detached biomass. As a result, it could be shown that biofilm structure, in terms of density and EPS volume, was largely influenced by hydrodynamic conditions. Furthermore, it was demonstrated that the EPS:bacteria ratio and distribution was largely influenced by substrate load. Finally, the characteristics in biofilm structure and development were reflected in the composition and quantity of the detached biomass.
在生物技术领域，生物膜和悬浮生物聚集体的组成对于系统性能或产品质量可能至关重要。因此，了解生物膜动力学对于任何工艺优化都很重要。本研究的目的是调查在不同水动力条件和不同葡萄糖负荷下生物膜的形成和脱落情况。共聚焦激光扫描显微镜被证明是一种快速的方法，可提供有关生物膜和脱落生物量中细菌和细胞外聚合物（EPS）的结构、分布和体积比的信息。结果表明，就密度和EPS体积而言，生物膜结构在很大程度上受水动力条件的影响。此外，还证明了EPS与细菌的比例和分布在很大程度上受底物负荷的影响。最后，生物膜结构和形成的特征反映在脱落生物量的组成和数量上。
