Shinagawa K, Yokoi R, Matsusaka N, Sugii S
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
J Vet Med Sci. 1991 Jun;53(3):419-22. doi: 10.1292/jvms.53.419.
Three murine monoclonal antibodies (MAbs) were prepared against an enterotoxin (ET) produced by Bacillus cereus. Although these MAbs were found to react with the ET, their specificities appeared to be different in competitive binding assays. One of the MAbs (D-8), which was highly reactive with the ET, will be useful in developing immunological methods to detect crude ET and to isolate the ET in high yield.
制备了三种针对蜡样芽孢杆菌产生的一种肠毒素（ET）的鼠单克隆抗体（MAb）。尽管发现这些单克隆抗体与该肠毒素发生反应，但在竞争结合试验中它们的特异性似乎有所不同。其中一种与该肠毒素反应强烈的单克隆抗体（D - 8），将有助于开发免疫方法来检测粗制肠毒素并高产率地分离该肠毒素。
