Yan Meijuan, Cheng Chun, Shao Xiaoyi, Qian Ji, Shen Aiguo, Xia Chunlin
Laboratory of Aging and Nervous Diseases & Cytoneurobiology Unit, Soochow University, Suzhou, 215123, PR China.
J Mol Histol. 2008 Jun;39(3):317-28. doi: 10.1007/s10735-008-9168-z. Epub 2008 Mar 5.
Glycosylation is one of the most important post-translational modifications. It is clear that the single step of beta-1,4-galactosylation is performed by a family of beta-1,4-galactosyltransferases (beta-1,4-GalTs), and that each member of this family may play a distinct role in different tissues and cells. beta-1,4-GalT I and V are involved in the biosynthesis of N-linked oligosaccharides. In the present study, Real-time PCR revealed that the beta-1,4-GalT I and V mRNAs reached peaks at 2 w after sciatic nerve crush. In situ hybridization showed that at 1 d after sciatic nerve crush, the expression levels of beta-1,4-GalT I and V mRNAs were strong at the crush site, and decreased gradually from crush site to the distal segments. In addition, combined in situ hybridization for beta1,4-GalT I and V mRNAs and immunohistochemistry for S100 showed that beta1,4-GalT I and V mRNAs were mainly located in Schwann cells. Lectin blot showed that the expression of Galbeta1,4GlcNAc group increased at 6 h immediately, reached a peak at 12 h and remained elevated up to 4 w after sciatic nerve crush. In conclusion, beta1,4-GalT I and V might play important roles in the regeneration of the injured sciatic nerve, and upregulation of Galbeta1,4GlcNAc group might be correlated with the process of the sciatic nerve injury.
糖基化是最重要的翻译后修饰之一。很明显，β-1,4-半乳糖基化的单个步骤是由β-1,4-半乳糖基转移酶（β-1,4-GalTs）家族执行的，并且该家族的每个成员可能在不同组织和细胞中发挥不同作用。β-1,4-GalT I和V参与N-连接寡糖的生物合成。在本研究中，实时PCR显示坐骨神经挤压后2周β-1,4-GalT I和V的mRNA达到峰值。原位杂交显示坐骨神经挤压后1天，β-1,4-GalT I和V的mRNA在挤压部位表达水平很强，并从挤压部位向远端逐渐降低。此外，β1,4-GalT I和V的mRNA原位杂交与S100免疫组化相结合显示，β1,4-GalT I和V的mRNA主要位于雪旺细胞中。凝集素印迹显示，Galβ1,4GlcNAc基团的表达在坐骨神经挤压后6小时立即增加，在12小时达到峰值，并在4周内一直保持升高。总之，β1,4-GalT I和V可能在坐骨神经损伤的再生中发挥重要作用，Galβ1,4GlcNAc基团的上调可能与坐骨神经损伤过程相关。
