Van Kruiningen H J, Ruiz B, Gumprecht L
Department of Pathobiology, University of Connecticut, Storrs 06269.
Can J Vet Res. 1991 Apr;55(2):199-202.
The susceptibility of young chickens to infection with an isolate of Mycobacterium paratuberculosis which had been recovered from diseased tissues of a patient with Crohn's disease was determined. Two-week-old Leghorn-Cochin chicks were inoculated with strain Linda. Six birds received 10(7) organisms orally, six intraperitoneally, and five intracardially. Six uninoculated birds served as contact controls. Birds from each group were necropsied at two-week intervals. Focal granulomatous lesions occurred in two of six chickens which were inoculated orally, in six of six inoculated intraperitoneally, in three of five inoculated intracardially, but in none of six controls. Of the 11 birds with lesions, acid-fast bacilli were demonstrated in five. Immunoperoxidase reactivity paralleled the presence of acid-fast bacilli.
测定了幼鸡对从一名克罗恩病患者病变组织中分离出的副结核分枝杆菌菌株感染的易感性。将两周龄的来航-科钦雏鸡接种琳达菌株。6只鸡经口接种10⁷个菌体，6只经腹腔接种，5只经心脏接种。6只未接种的鸡作为接触对照。每组鸡每隔两周进行剖检。经口接种的6只鸡中有2只出现局灶性肉芽肿病变，经腹腔接种的6只鸡全部出现病变，经心脏接种的5只鸡中有3只出现病变，而6只对照鸡均未出现病变。在11只有病变的鸡中，5只检测到抗酸杆菌。免疫过氧化物酶反应与抗酸杆菌的存在情况相符。
