MacKenzie L E, Erbe D V, Pulford K A, Lydyard P M, Fanger M W
Department of Immunology, U.C.M.S.M., London, U.K.
Immunol Lett. 1991 Jun;28(3):245-50. doi: 10.1016/0165-2478(91)90011-x.
There has been some controversy as to whether or not B cells can kill target cells through their Fc receptors. To address this, we have examined the ability of human B cells from a variety of sources to lyse hybridoma cells with specificity for either the B cell Fc gamma RII or Fc epsilon RII using a reverse killing assay, as well as their ability to lyse opsonized chicken erythrocytes using a classic ADCC assay. Tonsil B cells, chronic lymphocytic leukemia B cells, and Epstein-Barr virus-induced B cells, even after preactivation with a cocktail of cytokines, all failed to lyse any of these targets. We conclude that Fc gamma RII and Fc epsilon RII on human B cells are not cytotoxic trigger molecules.
关于B细胞是否能通过其Fc受体杀伤靶细胞一直存在一些争议。为了解决这个问题，我们使用反向杀伤试验检测了来自各种来源的人B细胞对具有B细胞FcγRII或FcεRII特异性的杂交瘤细胞进行裂解的能力，以及使用经典的抗体依赖的细胞介导的细胞毒性（ADCC）试验检测其裂解调理的鸡红细胞的能力。扁桃体B细胞、慢性淋巴细胞白血病B细胞和爱泼斯坦-巴尔病毒诱导的B细胞，即使在用细胞因子混合物预激活后，也都未能裂解任何这些靶细胞。我们得出结论，人B细胞上的FcγRII和FcεRII不是细胞毒性触发分子。
