确定肠道受损的绞痛马血液和腹腔液中血清素（5-羟色胺，5-HT）浓度升高的来源。

Determination of the source of increased serotonin (5-HT) concentrations in blood and peritoneal fluid of colic horses with compromised bowel.

作者信息

Delesalle C, van de Walle G R, Nolten C, Ver Donck L, van Hemelrijck A, Drinkenburg W, de Bosschere H, Claes P, Deprez P, Lefere L, Torfs S, Lefebvre R A

机构信息

Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

出版信息

Equine Vet J. 2008 Jun;40(4):326-31. doi: 10.2746/042516408X293583.

DOI:10.2746/042516408X293583

PMID:18321805

Abstract

REASONS FOR PERFORMING STUDY

Increased plasma (5-HT) concentrations are reported in horses predisposed to develop laminitis and after i.v. infusion of endotoxins. In the equine jejunum contractile 5-HT1A-like receptors show tachyphylaxia upon prolonged activation with 5-HT. Therefore, increased systemic 5-HT release in colic horses could play a possible role in the pathophysiology of ileus.

OBJECTIVE

To investigate possible increased systemic release of 5-HT in colic horses with compromised bowel and to identify the source of 5-HT overload.

METHODS

Concentrations of 5-HT were determined in plasma and peritoneal fluid (PF) of healthy horses (n = 10), strangulating small intestinal colic horses (n = 18), nonsurgical colic horses (n = 10) and cryptorchid stallions (n = 6). It was attempted to identify the source of 5-HT overload by comparing the blood and PF 5-HT concentrations within horses and by assessing the in vivo platelet activation through determination of the beta-thromboglobulin (beta-TG)/platelet factor 4 (PF4) ratio.

RESULTS

All horses in the strangulating small intestinal colic group had plasma (P = 0.006) and PF (P = 0.01) 5-HT concentrations above those found in the control group. Plasma beta-TG/PF4 ratio in these horses exceeded 2 in all cases, indicating in vivo platelet activation. Concentrations of 5-HT in PF of colic horses with compromised bowel were significantly lower than the corresponding plasma concentrations (P = 0.005).

POTENTIAL RELEVANCE

In horses with compromised bowel, significant amounts of 5-HT can be released into the systemic circulation, through massive release of platelet-stored 5-HT. 5-HT is a very potent proinflammatory, vasoconstrictive and immunomodulatory agent. In view of the rapid and prolonged tachyphylaxia, shown for the jejunal 5-HT1A-like receptors, this increased systemic 5-HT release could play a role in the pathophysiology of ileus in horses.

摘要

开展本研究的原因

据报道，易患蹄叶炎的马匹以及静脉输注内毒素后的马匹血浆中5-羟色胺（5-HT）浓度会升高。在马的空肠中，收缩性5-HT1A样受体在被5-HT长期激活后会出现快速减敏。因此，患急腹症的马体内5-HT系统释放增加可能在肠梗阻的病理生理学中发挥作用。

目的

研究肠道受损的患急腹症的马体内5-HT系统释放是否可能增加，并确定5-HT过载的来源。

方法

测定了健康马（n = 10）、绞窄性小肠梗阻马（n = 18）、非手术性急腹症马（n = 10）和隐睾种马（n = 6）血浆和腹腔液（PF）中的5-HT浓度。通过比较马体内血液和PF中的5-HT浓度，并通过测定β-血小板球蛋白（β-TG）/血小板因子4（PF4）比值来评估体内血小板活化情况，试图确定5-HT过载的来源。