Pihl Tina Holberg, Andersen Pia Haubro, Kjelgaard-Hansen Mads, Mørck Nina Brinch, Jacobsen Stine
Department of Large Animal Sciences, Large Animal Surgery, Taastrup, Denmark.
Vet Clin Pathol. 2013 Jun;42(2):177-83. doi: 10.1111/vcp.12031. Epub 2013 Apr 11.
Peritoneal fluid (PF) analysis is a valuable diagnostic tool in equine medicine. Markers such as serum amyloid A (SAA) and haptoglobin (Hp) could facilitate the diagnosis of inflammatory abdominal conditions.
The objectives were to (1) establish reference intervals (RI) for SAA and Hp in serum and PF in healthy horses, (2) compare SAA and Hp concentrations between healthy horses and horses with colic, and (3) to assess the correlation between serum and PF concentrations.
Serum amyloid A and Hp concentrations were determined by automated assays in prospectively enrolled healthy reference horses and horses with colic. RIs were calculated, group concentrations were compared by Student's t-test, and Pearson's correlation for serum and PF concentrations were determined.
In healthy horses (n = 62) the measurements for SAA were below the detection limit (0.5 mg/L) in 94% of serum samples and 98% of PF samples. Horses with colic (n = 61) had statistically significantly increased SAA concentrations in serum (P < .0001) and PF (P = .0013). While PF Hp concentrations were increased in horses with colic the serum concentrations of Hp were decreased (P < .0001). There was a strong correlation between paired serum and PF SAA concentrations (n = 94, R = .72, P < .0001), whereas the correlation between paired serum and PF Hp was weak (n = 94, R = .22, P = .0382). Finally, horses with colic tended to have serum SAA and PF Hp concentrations above the RIs.
With the apparent difference between healthy horses and horses with colic and the presently established RIs, serum SAA and PF Hp concentrations represent potential valuable diagnostic markers for inflammatory abdominal conditions in that species.
腹腔积液（PF）分析是马医学中一种有价值的诊断工具。血清淀粉样蛋白A（SAA）和触珠蛋白（Hp）等标志物有助于诊断腹部炎症性疾病。
本研究目的为：（1）建立健康马匹血清和腹腔积液中SAA和Hp的参考区间（RI）；（2）比较健康马匹和患绞痛马匹之间SAA和Hp的浓度；（3）评估血清和腹腔积液浓度之间的相关性。
通过自动检测法测定前瞻性纳入的健康对照马匹和患绞痛马匹的血清淀粉样蛋白A和Hp浓度。计算参考区间，采用Student t检验比较组间浓度，并确定血清和腹腔积液浓度的Pearson相关性。
在健康马匹（n = 62）中，94%的血清样本和98%的腹腔积液样本中SAA测量值低于检测限（0.5 mg/L）。患绞痛的马匹（n = 61）血清（P <.0001）和腹腔积液（P =.0013）中SAA浓度在统计学上显著升高。虽然患绞痛马匹的腹腔积液Hp浓度升高，但血清Hp浓度降低（P <.0001）。配对的血清和腹腔积液SAA浓度之间存在强相关性（n = 94，R =.72，P <.0001），而配对的血清和腹腔积液Hp之间的相关性较弱（n = 94，R =.22，P =.0382）。最后，患绞痛的马匹血清SAA和腹腔积液Hp浓度往往高于参考区间。
鉴于健康马匹和患绞痛马匹之间存在明显差异以及目前建立的参考区间，血清SAA和腹腔积液Hp浓度是该物种腹部炎症性疾病潜在的有价值诊断标志物。
