Griesinger F, Younger P W, Kersey J H
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis.
Leukemia. 1991 Aug;5(8):673-9.
To gain a better understanding of the organization of the complex T-cell antigen receptor alpha/delta (TCR alpha/delta) locus, a deletional analysis using the known six variable (V) regions of the TCR delta was performed in informative leukemic cell lines and fresh leukemias. We and others have previously reported a high incidence of V delta 2-(D)-D delta 3 rearrangements in non-T, non-B-lymphoid precursor acute lymphocytic leukemia (LP-ALL). In contrast V delta 4, V delta 5, V delta 6 rearrangements were rare or absent. V delta-J alpha rearrangements were found in LP-ALL and in T-ALL. Our deletion and rearrangement data combined with that of others suggest the following 5' to 3' organization of the TCR alpha/delta locus: V delta 6-(V delta 4-V alpha 1.2)-V alpha 12.1-V alpha 13.1-V delta 1-V delta 17.1-V delta 5-delta Rec-V delta 2-D/J/C delta-V delta 3-TEA-psi J alpha-J alpha G. The frequency of rearrangements of the various V delta genes suggests preferential use of the V delta most proximal to D/J delta.
为了更好地理解复杂的T细胞抗原受体α/δ（TCRα/δ）基因座的组织方式，我们在信息丰富的白血病细胞系和新鲜白血病样本中，利用已知的TCRδ六个可变（V）区域进行了缺失分析。我们和其他人之前曾报道，在非T、非B淋巴细胞前体急性淋巴细胞白血病（LP-ALL）中，Vδ2-（D）-Dδ3重排的发生率很高。相比之下，Vδ4、Vδ5、Vδ6重排很少见或不存在。在LP-ALL和T-ALL中发现了Vδ-Jα重排。我们的缺失和重排数据与其他人的数据相结合，提示了TCRα/δ基因座从5'到3'的如下组织方式：Vδ6-（Vδ4-Vα1.2）-Vα12.1-Vα13.1-Vδ1-Vδ17.1-Vδ5-δRec-Vδ2-D/J/Cδ-Vδ3-TEA-ψJα-JαG。各种Vδ基因重排的频率表明，最靠近D/Jδ的Vδ被优先使用。
