Huang Junsheng, Li Xiutao, Zheng Yanhui, Zhang Yong, Zhao Ruiying, Gao Xicai, Yan Husheng
Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
Macromol Biosci. 2008 Jun 11;8(6):508-15. doi: 10.1002/mabi.200700256.
Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe(3)O(4) nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush-grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.
通过原子转移自由基聚合（ATRP），由含有分散的Fe(3)O(4)纳米颗粒的聚合物微球进行接枝无规共聚，制备了聚[（甲基丙烯酸缩水甘油酯）-co-（单甲基甘油丙烯酸酯）]接枝磁性微球。将青霉素G酰化酶（PGA）固定在聚合物刷接枝的磁性微球上。初始甲基丙烯酸缩水甘油酯/单甲基甘油丙烯酸酯比例为40/60至60/40制备的固定化PGA比在初始单体混合物中含有更高比例甲基丙烯酸缩水甘油酯制备的固定化PGA具有更高的催化活性。固定化PGA表现出高的热稳定性和对pH变化增强的耐受性。
