Svenson M, Kayser L, Hansen M B, Rasmussen A K, Bendtzen K
Department of Infectious Diseases M, Rigshospitalet University Hospital, Copenhagen, Denmark.
Cytokine. 1991 Mar;3(2):125-30. doi: 10.1016/1043-4666(91)90032-9.
Cellular binding of interleukin-1 (IL-1) was tested on monolayers of human thyrocytes in secondary culture, on long-term cultures of human thyrocytes, and on the rat thyroid cell line FRTL-5. The human thyrocytes in secondary culture showed specific binding of human 125I-rIL-1 alpha. Scatchard plots of data obtained at 4 degrees C indicated the presence of a single population of receptors with a Kd of 30 to 170 pM and 2,000 to 6,000 receptors per cell. Incubation at room temperature resulted in internalization of the receptor-ligand complex. Parallel experiments were performed with the IL-1 receptor-positive murine T-cell lines EL-4 and NOB-1. The IL-1 receptors on these cells had Kd values one fifth to one tenth those on human thyroid cells in secondary culture. Both rIL-1 alpha and rIL-1 beta inhibited 125I-rIL-1 alpha binding to human thyrocytes and the murine T cells. In contrast to the cells in secondary culture, there was no specific binding of 125I-rIL-1 alpha to long-term cultivated human thyroid cells or to the FRTL-5 cells. We concluded that recently described differences in the response to IL-1 of different thyroid cell culture systems are most likely caused by differences in expression of IL-1 receptors.
在人甲状腺细胞的二次培养单层、人甲状腺细胞的长期培养物以及大鼠甲状腺细胞系FRTL - 5上测试了白细胞介素 - 1（IL - 1）的细胞结合情况。人甲状腺细胞的二次培养物显示出对人125I - rIL - 1α的特异性结合。在4℃下获得的数据的Scatchard图表明存在单一受体群体，其解离常数（Kd）为30至170 pM，每个细胞有2000至6000个受体。在室温下孵育导致受体 - 配体复合物内化。用IL - 1受体阳性的小鼠T细胞系EL - 4和NOB - 1进行了平行实验。这些细胞上的IL - 1受体的Kd值是二次培养的人甲状腺细胞上Kd值的五分之一至十分之一。rIL - 1α和rIL - 1β均抑制125I - rIL - 1α与人甲状腺细胞和小鼠T细胞的结合。与二次培养的细胞不同，125I - rIL - 1α与长期培养的人甲状腺细胞或FRTL - 5细胞没有特异性结合。我们得出结论，最近描述的不同甲状腺细胞培养系统对IL - 1反应的差异很可能是由IL - 1受体表达的差异引起的。
