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与DNA结合的人类Brn-5转录因子的结晶及初步X射线分析。

Crystallization and preliminary X-ray analysis of human Brn-5 transcription factor in complex with DNA.

作者信息

Pereira Jose Henrique, Ha Sung Chul, Kim Sung-Hou

机构信息

Department of Chemistry, University of California, Berkeley, California 94720, USA.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):175-8. doi: 10.1107/S1744309108003370. Epub 2008 Feb 23.

DOI:10.1107/S1744309108003370
PMID:18323601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2374152/
Abstract

The Brn-5 protein plays an important role in the control of cellular development and belongs to a class of transcription factors that usually contain two domains: the POU homeodomain (POU(HD)) and the POU-specific domain (POU(S)). Since high-quality crystals suitable for crystallographic studies of the proteins of this class are difficult to obtain, all the known structural information available is for POU(HD) and/or POU(S). This paper describes several critical steps that allowed the production of high-quality crystals of the full-length Brn-5 protein complexed with its cognate DNA.

摘要

Brn-5蛋白在细胞发育控制中发挥着重要作用，属于一类转录因子，这类转录因子通常包含两个结构域：POU同源结构域（POU(HD)）和POU特异性结构域（POU(S)）。由于难以获得适用于这类蛋白质晶体学研究的高质量晶体，所有已知的结构信息都来自POU(HD)和/或POU(S)。本文描述了几个关键步骤，这些步骤使得能够产生与同源DNA复合的全长Brn-5蛋白的高质量晶体。

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本文引用的文献

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[10] Dynamic light scattering in evaluating crystallizability of macromolecules.[10] 动态光散射在评估大分子结晶性中的应用
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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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Involvement of mPOU (Brn-5), a class VI POU protein, in the gene expression of Pit-1 as well as PRL.VI类POU蛋白mPOU（Brn-5）参与Pit-1以及催乳素的基因表达。
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Crystallization of redox-insensitive Oct1 POU domain with different DNA-response elements.具有不同DNA反应元件的氧化还原不敏感Oct1 POU结构域的结晶
Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1634-8. doi: 10.1107/s090744490101099x. Epub 2001 Oct 25.
5
The POU gene Brn-5 is induced by neuregulin and is restricted to myelinating Schwann cells.POU基因Brn-5由神经调节蛋白诱导产生，且仅限于有髓鞘的施万细胞。
Mol Cell Neurosci. 2001 Apr;17(4):683-95. doi: 10.1006/mcne.2000.0957.
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Mol Cell Biol. 1997 May;17(5):2391-400. doi: 10.1128/MCB.17.5.2391.
8
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