蜡样芽孢杆菌肽聚糖N - 乙酰葡糖胺脱乙酰酶BC1960在其底物(GlcNAc)6存在下的纯化、结晶及初步X射线分析
Purification, crystallization and preliminary X-ray analysis of the peptidoglycan N-acetylglucosamine deacetylase BC1960 from Bacillus cereus in the presence of its substrate (GlcNAc)6.
作者信息
Tsalafouta Aleka, Psylinakis Emmanuel, Kapetaniou Evangelia G, Kotsifaki Dina, Deli Alexandra, Roidis Alexandros, Bouriotis Vasilis, Kokkinidis Michael
机构信息
Department of Biology, University of Crete, PO Box 2208, GR-71110, Heraklion, Crete, Greece.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Mar 1;64(Pt 3):203-5. doi: 10.1107/S1744309108002510. Epub 2008 Feb 29.
Abstract
The peptidoglycan N-acetylglucosamine (GlcNAc) deacetylase BC1960 from Bacillus cereus (EC 3.5.1.33), an enzyme consisting of 275 amino acids, was crystallized in the presence of its substrate (GlcNAc)(6). The crystals belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 92.7, c = 242.9 A and four molecules in the asymmetric unit. A complete data set was collected at 100 K to a resolution of 2.38 A using synchrotron radiation.
摘要
蜡样芽孢杆菌的肽聚糖N - 乙酰葡糖胺（GlcNAc）脱乙酰酶BC1960（EC 3.5.1.33）是一种由275个氨基酸组成的酶，在其底物（GlcNAc）6存在的情况下结晶。晶体属于四方晶系空间群P41212，晶胞参数a = b = 92.7，c = 242.9 Å，不对称单位中有四个分子。使用同步辐射在100 K下收集了完整的数据集，分辨率达到2.38 Å。
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