Sakuma Motoki, Kubota Shunichiro
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Tokyo 153-8902, Japan.
Biosci Biotechnol Biochem. 2008 Mar;72(3):872-6. doi: 10.1271/bbb.70612. Epub 2008 Mar 7.
In many organisms, glycogen gives rise to 1,5-anhydro-D-fructose (AF), which is reduced to 1,5-anhydro-D-glucitol (AG). AF reductase, which catalyzes the latter reaction, was purified from pig liver, but mouse ortholog has not yet been reported. In the database, aldo-keto reductase family 1, member E1 (AKR1E1) showed highest homology to pig enzyme. We confirmed that cloned AKR1E1 is mouse ortholog based on enzymatic properties of purified recombinant protein.
在许多生物体中，糖原会产生1,5-脱水-D-果糖（AF），后者会被还原为1,5-脱水-D-葡萄糖醇（AG）。催化后一反应的AF还原酶已从猪肝中纯化出来，但小鼠同源物尚未见报道。在数据库中，醛酮还原酶家族1成员E1（AKR1E1）与猪酶的同源性最高。基于纯化重组蛋白的酶学性质，我们证实克隆的AKR1E1是小鼠同源物。
