猪和牛肠肽酶对肽Val-(Asp)4-Lys-Ile-Val-Gly及其类似物的底物特异性。

Substrate specificities of porcine and bovine enteropeptidases toward the peptide Val-(Asp)4-Lys-Ile-Val-Gly and its analogs.

作者信息

Kim Yong-Tae, Nishii Wataru, Matsushima Masashi, Inoue Hideshi, Ito Hisashi, Park Sun Joo, Takahashi Kenji

机构信息

Protein Research Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.

出版信息

Biosci Biotechnol Biochem. 2008 Mar;72(3):905-8. doi: 10.1271/bbb.70732. Epub 2008 Mar 7.

DOI:10.1271/bbb.70732

PMID:18323639

Abstract

The substrate specificities of porcine and bovine enteropeptidases were investigated using the peptide Val-(Asp)(4)-Lys-Ile-Val-Gly and its various analogs with mutations in the (Asp)(4)-Lys sequence as substrates. The results indicated that in addition to P1 Lys, P2 Asp in the substrates is most important, that P3 Asp is additionally important, and that P5 Asp contributes somewhat to the susceptibility, and that P4 Asp is the least important. These results were essentially identical as between porcine and bovine enteropeptidases.

摘要

以肽Val-(Asp)₄-Lys-Ile-Val-Gly及其在(Asp)₄-Lys序列中具有突变的各种类似物作为底物，研究了猪和牛肠肽酶的底物特异性。结果表明，除了底物中的P1 Lys外，P2 Asp最为重要，P3 Asp也很重要，P5 Asp对敏感性有一定贡献，而P4 Asp最不重要。这些结果在猪和牛肠肽酶之间基本相同。