Mizuno Ryoji, Itoh Yoshikane, Nishizawa Yoko, Kezuka Yuichiro, Suzuki Kazushi, Nonaka Takamasa, Watanabe Takeshi
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, 8050 Ikarashi-2, Niigata 950-2181, Japan.
Biosci Biotechnol Biochem. 2008 Mar;72(3):893-5. doi: 10.1271/bbb.70693. Epub 2008 Mar 7.
To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.
为了确定来自水稻品种日本晴（粳稻亚种）的19家族几丁质酶OsChia1b的性质和结构，在大肠杆菌细胞中表达了重组OsChia1b，并通过几丁质亲和柱色谱法将其纯化至同质。OsChia1b对可溶性几丁质底物具有高活性，但对结晶几丁质无活性，并且能明显抑制里氏木霉的菌丝延伸。
