Claesson M H, Rudolphi A, Tscherning T, Reimann J
Department of Medical Anatomy A, PANUM Institute, University of Copenhagen.
Eur J Immunol. 1991 Sep;21(9):2057-62. doi: 10.1002/eji.1830210913.
Intraperitoneal injection of 2 x 10(7) nonfractionated spleen cells (SC) from C57BL/6 (B6, H-2b) mice into completely allogeneic immunodeficient H-2d scid mice induced clinical and histological signs of acute graft-vs.-host disease (GVHD), with all transplanted severe combined immunodeficiency (scid) mice dying in the 3rd week post-transfer. In contrast four out of five scid mice survived for greater than 7 weeks after intravenous (i.v.) injections of equal numbers of B6 SC. Intravenously allotransplanted scid mice analyzed in the 8th week post-transfer had engrafted donor-type CD4+ and CD8+ T cells in the spleens but showed no clinical or histological evidence of GVHD. i.v. injection of 10(7) or 10(6)O B6 SC engrafted allogeneic T cells in spleens of scid recipients; in contrast, i.v. injection of 10(5) nonfractionated B6 SC or 3 x 10(5) cell sorter-purified, naive or anti-H-2d-primed splenic CD4+ or CD8+ B6 T cells led to rejection by young scid recipient mice. B6 T cells engrafted into spleens of scid mice after i.v. injection showed proliferative anti-host alloreactivity in vitro. No cytotoxic reactivity against host-type alloantigens was found in standard 4-h 51Cr-release assays. These data demonstrate that allogeneic T cells injected i.v. into immunodeficient scid mice are partially tolerized against host-type alloantigens.
将来自C57BL/6（B6，H-2b）小鼠的2×10⁷个未分级脾细胞（SC）腹腔注射到完全异基因的免疫缺陷H-2d scid小鼠中，诱发了急性移植物抗宿主病（GVHD）的临床和组织学症状，所有移植的严重联合免疫缺陷（scid）小鼠在移植后第3周死亡。相比之下，五只scid小鼠中有四只在静脉注射等量的B6 SC后存活超过7周。在移植后第8周分析的静脉内同种异体移植scid小鼠，其脾脏中植入了供体型CD4⁺和CD8⁺ T细胞，但未显示出GVHD的临床或组织学证据。静脉注射10⁷或10⁶个B6 SC可使scid受体的脾脏中植入同种异体T细胞；相反，静脉注射10⁵个未分级的B6 SC或3×10⁵个经细胞分选纯化的、未致敏或抗H-2d预致敏的脾脏CD4⁺或CD8⁺ B6 T细胞会导致年轻的scid受体小鼠发生排斥反应。静脉注射后植入scid小鼠脾脏的B6 T细胞在体外显示出增殖性抗宿主同种异体反应性。在标准的4小时⁵¹Cr释放试验中未发现针对宿主型同种异体抗原的细胞毒性反应。这些数据表明，静脉注射到免疫缺陷scid小鼠体内的同种异体T细胞对宿主型同种异体抗原产生了部分耐受。
