Kauma S W, Aukerman S L, Eierman D, Turner T
Department of Obstetrics and Gynecology, Medical College of Virginia, Richmond 23298.
J Clin Endocrinol Metab. 1991 Oct;73(4):746-51. doi: 10.1210/jcem-73-4-746.
Colony-stimulating factor-1 (CSF-1), a growth factor produced by monocytes, macrophages, fibroblasts, and endothelial cells, has been implicated in the functional regulation and growth of the murine placenta through the presence of the CSF-1 receptor, c-fms, found in this tissue. In this study we examined the tissue levels of CSF-1 by RIA and the relative expression of CSF-1 and c-fms mRNA by Northern blot analysis in human endometrial, decidual, and placental tissues during the normal menstrual cycle and early pregnancy. All endometrial, decidual, and placental tissues demonstrated extractable immunoreactive CSF-1 and expressed the 4.0-kilobase CSF-1 mRNA species. First trimester decidual tissue expressed higher levels of CSF-1 mRNA than proliferative (3.2-fold higher; P less than 0.01) or secretory (2.4-fold higher; P less than 0.01) endometrial tissues, whereas proliferative and secretory endometrial tissues expressed similar levels of CSF-1 mRNA. Tissue extractable levels of immunoreactive CSF-1 were 3.2-fold (P less than 0.05) higher in first trimester decidual tissue and 2.9-fold (P less than 0.05) higher in secretory endometrial tissue compared to levels in proliferative endometrial tissue, whereas first trimester decidua and secretory endometrial tissues had similar levels of immunoreactive CSF-1. There was expression of c-fms mRNA in all endometrial and first trimester decidual tissue samples, with little change during the menstrual cycle and early pregnancy. In placenta, there was a positive correlation of increasing CSF-1 and c-fms mRNA expression with increasing gestational age. These results suggest that there is increased local production of CSF-1 in tissues found at the maternal-fetal interface during the time of implantation and early pregnancy. This increased production of CSF-1 may play a role in decidual function and placental growth through the presence of c-fms in these tissues.
集落刺激因子-1（CSF-1）是一种由单核细胞、巨噬细胞、成纤维细胞和内皮细胞产生的生长因子，通过在该组织中发现的CSF-1受体c-fms参与小鼠胎盘的功能调节和生长。在本研究中，我们通过放射免疫分析（RIA）检测了人子宫内膜、蜕膜和胎盘组织中CSF-1的组织水平，并通过Northern印迹分析检测了正常月经周期和早孕期间CSF-1和c-fms mRNA的相对表达。所有子宫内膜、蜕膜和胎盘组织均显示可提取的免疫反应性CSF-1，并表达4.0千碱基的CSF-1 mRNA种类。孕早期蜕膜组织表达的CSF-1 mRNA水平高于增殖期（高3.2倍；P<0.01）或分泌期（高2.4倍；P<0.01）子宫内膜组织，而增殖期和分泌期子宫内膜组织表达的CSF-1 mRNA水平相似。与增殖期子宫内膜组织相比，孕早期蜕膜组织中免疫反应性CSF-1的组织可提取水平高3.2倍（P<0.05），分泌期子宫内膜组织中高2.9倍（P<0.05），而孕早期蜕膜和分泌期子宫内膜组织的免疫反应性CSF-1水平相似。所有子宫内膜和孕早期蜕膜组织样本中均有c-fms mRNA表达，在月经周期和早孕期间变化不大。在胎盘中，CSF-1和c-fms mRNA表达的增加与孕周的增加呈正相关。这些结果表明，在着床和早孕期间，母胎界面组织中CSF-1的局部产生增加。CSF-1的这种增加产生可能通过这些组织中c-fms的存在，在蜕膜功能和胎盘生长中发挥作用。
