Trindade Sergio Henrique Kiemle, Macchione Mariângela, Guimarães Eliane Tigre, Trindade Inge Elly Kiemle, Saldiva Paulo Hilário Nascimento, Lorenzi-Filho Geraldo
Laboratory of Experimental Air Pollution, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Int J Pediatr Otorhinolaryngol. 2008 May;72(5):581-5. doi: 10.1016/j.ijporl.2008.01.003. Epub 2008 Mar 5.
Children with cleft palate (CP) have a high prevalence of sinusitis. Considering that nasal mucus properties play a pivotal role in the upper airway defense mechanism, the aim of the study was to evaluate nasal mucus transportability and physical properties from children with CP.
Hospital for Rehabilitation of Craniofacial Anomalies, School of Dentistry, University of São Paulo, Bauru, SP, Brazil and Laboratory of Experimental Air Pollution, School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Nasal mucus samples were collected by nasal aspiration from children with CP and without CP (non-CP). Sneeze clearance (SC) was evaluated by the simulated sneeze machine. In vitro mucus transportability (MCT) by cilia was evaluated by the frog palate preparation. Mucus physical surface properties were assessed by measuring the contact angle (CA). Mucus rheology was determined by means of a magnetic rheometer, and the results were expressed as log G* (vectorial sum of viscosity and elasticity) and tan delta (relationship between viscosity and elasticity) measured at 1 and 100 rad/s.
Mucus samples from children with CP had a higher SC than non-CP children (67+/-30 and 41+/-24 mm, respectively, p<0.05). Mucus samples from children with CP had a lower CA (24+/-16 degrees and 35+/-11 degrees , p<0.05) and a higher tan delta 100 (0.79+/-0.24 and 0.51+/-0.12, p<0.05) than non-CP children. There were no significant differences in mucus MCT, log G* 1, tan delta 1 and log G* 100 obtained for CP and non-CP children.
Nasal mucus physical properties from children with CP are associated with higher sneeze transportability. The high prevalence of sinusitis in children with CP cannot be explained by changes in mucus physical properties and transportability.
腭裂患儿鼻窦炎的患病率较高。鉴于鼻黏液特性在上呼吸道防御机制中起关键作用，本研究旨在评估腭裂患儿鼻黏液的运输能力和物理特性。
巴西圣保罗州鲍鲁市圣保罗大学牙科学院颅面畸形康复医院以及巴西圣保罗州圣保罗市圣保罗大学医学院实验空气污染实验室。
通过鼻腔抽吸收集腭裂患儿和非腭裂患儿（非CP）的鼻黏液样本。使用模拟喷嚏机评估喷嚏清除率（SC）。通过蛙腭制备评估纤毛介导的体外黏液运输能力（MCT）。通过测量接触角（CA）评估黏液的物理表面特性。使用磁流变仪测定黏液流变学，结果以1和100 rad/s下测量的log G*（黏度和弹性的矢量和）和tanδ（黏度与弹性的关系）表示。
腭裂患儿的黏液样本SC高于非腭裂患儿（分别为67±30和41±24 mm，p<0.05）。腭裂患儿的黏液样本CA低于非腭裂患儿（24±16°和35±11°，p<0.05），tanδ100高于非腭裂患儿（0.79±0.24和0.51±0.12，p<0.05）。腭裂患儿和非腭裂患儿的黏液MCT、log G* 1、tanδ1和log G* 100无显著差异。
腭裂患儿的鼻黏液物理特性与较高的喷嚏运输能力相关。腭裂患儿鼻窦炎的高患病率不能用黏液物理特性和运输能力的变化来解释。
