García-de-la-Fuente Celia, Campo-Esquisabel Ana Belén, Unda Fabián, Ruiz de Alegría Carlos, Benito Natividad, Martínez-Martínez Luis
Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Spain.
Diagn Microbiol Infect Dis. 2008 Jun;61(2):232-4. doi: 10.1016/j.diagmicrobio.2008.01.020. Epub 2008 Mar 5.
Detection of Arcanobacterium haemolyticum is based upon typical beta-hemolysis and colony morphology, but it may go undetected if only conventional sheep blood agar media for detection of beta-hemolytic streptococci are used. The influence of different culture media, atmospheres, and times of incubation for the recognition of colonies of 47 isolates of A. haemolyticum was studied. After 48 h of incubation, trypticase soy agar with 5% horse blood in 5% CO(2) was the best medium.
溶血隐秘杆菌的检测基于典型的β溶血和菌落形态，但如果仅使用用于检测β溶血性链球菌的传统绵羊血琼脂培养基，可能无法检测到该菌。研究了不同培养基、培养环境和培养时间对47株溶血隐秘杆菌菌落识别的影响。培养48小时后，含5%马血的胰蛋白酶大豆琼脂在5%二氧化碳环境中是最佳培养基。
