Zhang Jian-shui, Liu Yong, Xu Jie-hua, Yang Peng-bo, Xiao Xin-li, Chen Xin-lin, Tian Yu-mei, Zhang Jun-feng
Institute of Neurobiology,School of Medicine, Xioan Jiaotong University, Xi'an, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Feb;33(2):99-102.
To prove the existence neurons in the rat corpus callosum, the potential function of these neurons and their connection.
Immunohistochemistry was used performed to examine the expressions of NeuN, a mature neuron marker,and N-type voltage-dependent valcium channel alpha1-subunit (Cav2.2)in the section of the rat corpus callosum. Horseradish peroxidase (HRP) normal sodium solution (30%), the retrograde tracer,was injected under the frontal forceps of corpus callousm and HRP absorbed by the process of neurons in the brain slices was stained with tetramethyl benzidine.
There were some NeuN positive cells in the rat corpus callosum and Cav2.2 was detected in some of these NeuN positive cells.Neurons with positive HRP were found in the rat corpus callosum and some of these neurons connected to the cortex or corpus striatum.
There are a few neurons in the corpus callosum of adult rats and some of them express Cav2.2. Neurons in the corpus callosum have connections with the brain cortex or corpora striatum.
证实大鼠胼胝体中神经元的存在、这些神经元的潜在功能及其连接。
采用免疫组织化学法检测大鼠胼胝体切片中成熟神经元标志物NeuN和N型电压依赖性钙通道α1亚基（Cav2.2）的表达。将逆行示踪剂辣根过氧化物酶（HRP）生理盐水溶液（30%）注入胼胝体额钳下方，并用四甲基联苯胺对脑片中被神经元突起吸收的HRP进行染色。
大鼠胼胝体中有一些NeuN阳性细胞，部分NeuN阳性细胞中检测到Cav2.2。在大鼠胼胝体中发现了HRP阳性神经元，其中一些神经元与皮质或纹状体相连。
成年大鼠胼胝体中存在少量神经元，部分神经元表达Cav2.2。胼胝体中的神经元与大脑皮质或纹状体有连接。
