具有修饰色氨酸残基的非核糖体脂肽的营养缺陷型前体定向生物合成。
Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues.
作者信息
Amir-Heidari Bagher, Thirlway Jenny, Micklefield Jason
机构信息
School of Chemistry and Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester, UK.
出版信息
Org Biomol Chem. 2008 Mar 21;6(6):975-8. doi: 10.1039/b718766c. Epub 2008 Feb 11.
Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolor Trp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action.
向天蓝色链霉菌色氨酸营养缺陷型菌株WH101投喂5-羟基色氨酸和5-氟色氨酸，会产生多种具有修饰色氨酸残基的新型钙依赖性抗生素（CDA）。预计该方法可用于调节含色氨酸的非核糖体肽天然产物的生物学特性，或生成具有有用荧光特性的类似物，以研究生物学作用机制。
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