Almeida Lana Carneiro, Tomita Luciana Yuki, D'Almeida Vânia, Cardoso Marly Augusto
Faculdade de Saúde Pública, Universidade de São Paulo, Sço Paulo, Brasil.
Cad Saude Publica. 2008 Mar;24(3):587-96. doi: 10.1590/s0102-311x2008000300012.
This study examined the socio-demographic, lifestyle, gynecological, and obstetric factors associated with serum or plasma concentrations of homocysteine, folic acid, and vitamins B12 and B6 among low-income women in São Paulo, Brazil. Serum concentrations of folic acid and vitamin B12 were measured by fluoroimmunoassay, while plasma vitamin B6 and homocysteine levels were measured by reversed-phase high performance liquid chromatography. Independent variables were initially selected by Pearson correlation or Kruskal-Wallis test (p < 0.20). Based on cut-off values, altered concentrations of homocysteine, folic acid, and vitamins B12 and B6 were found in 20%, 6%, 11%, and 67% of participants, respectively. Age was positively correlated with vitamin B6 and homocysteine plasma concentrations (p < 0.001). Body mass index was positively correlated with vitamin B6 plasma concentration (p < 0.001). Multiple linear regression models accounted for 10.2%, 5.8%, 14.4%, and 9.4% of folic acid, vitamins B12 and B6, and homocysteine plasma or serum concentrations, respectively. In this study, socio-demographic, lifestyle, gynecological, and obstetric variables showed important predictive value for serum or plasma levels of the biochemical indicators assessed.
本研究调查了巴西圣保罗低收入女性中与血清或血浆同型半胱氨酸、叶酸、维生素B12和维生素B6浓度相关的社会人口学、生活方式、妇科和产科因素。叶酸和维生素B12的血清浓度通过荧光免疫测定法测量，而血浆维生素B6和同型半胱氨酸水平通过反相高效液相色谱法测量。自变量最初通过Pearson相关性或Kruskal-Wallis检验进行选择（p<0.20）。根据临界值，分别在20%、6%、11%和67%的参与者中发现同型半胱氨酸、叶酸、维生素B12和维生素B6的浓度发生改变。年龄与维生素B6和血浆同型半胱氨酸浓度呈正相关（p<0.001）。体重指数与血浆维生素B6浓度呈正相关（p<0.001）。多元线性回归模型分别解释了叶酸、维生素B12和维生素B6以及同型半胱氨酸血浆或血清浓度的10.2%、5.8%、14.4%和9.4%。在本研究中，社会人口学、生活方式、妇科和产科变量对所评估的生化指标的血清或血浆水平显示出重要的预测价值。
