Péter Gábor, Tornai-Lehoczki Judit, Dlauchy Dénes
National Collection of Agricultural and Industrial Microorganisms, Corvinus University of Budapest, Faculty of Food Sciences, Somlói út 14-16, 1118 Budapest, Hungary.
Antonie Van Leeuwenhoek. 2008 Aug;94(2):217-22. doi: 10.1007/s10482-008-9234-2. Epub 2008 Mar 10.
Three methanol-assimilating, nitrate-negative yeast strains representing a hitherto undescribed species, were isolated from leaf and rotten wood samples collected in temperate forests in Hungary. Analysis of the D1/D2 large subunit ribosomal RNA gene sequences placed the strains in the Ogataea clade. The three strains share identical D1/D2 and ITS sequences and significantly differ from the genetically most closely related species, Pichia pilisensis. Five substitutions in D1/D2 and more than 10% difference in the ITS regions were detected. A novel yeast species, Ogataea nitratoaversa, is proposed to accommodate these isolates. The type culture is NCAIM Y.01837(T) (CBS 10,796, NRRL Y-48,449). As the current description of the genus does not allow the inclusion of nitrate negative species, the emendation of the diagnosis of the genus Ogataea Yamada, Maeda and Mikata is proposed.
从匈牙利温带森林采集的叶片和朽木样本中分离出三株同化甲醇、硝酸盐阴性的酵母菌株，它们代表了一个迄今未被描述的物种。对D1/D2大亚基核糖体RNA基因序列的分析将这些菌株归入Ogataea分支。这三株菌株具有相同的D1/D2和ITS序列，与遗传关系最密切的物种Pichia pilisensis有显著差异。在D1/D2区域检测到五个替换，ITS区域差异超过10%。提议将这些分离株归为一个新的酵母物种，即反硝化奥默酵母（Ogataea nitratoaversa）。模式菌株为NCAIM Y.01837(T)（CBS 10,796，NRRL Y-48,449）。由于目前该属的描述不允许纳入硝酸盐阴性物种，因此提议对山田、前田和三方所定义的Ogataea属的诊断进行修订。
