Dedepsidis Evaggelos, Pliaka Vaia, Kyriakopoulou Zaharoula, Brakoulias Christos, Levidiotou-Stefanou Stamatina, Pratti Anastassia, Mamuris Zissis, Markoulatos Panayotis
Department of Biochemistry and Biotechnology, University of Thessaly, Greece.
FEMS Immunol Med Microbiol. 2008 Apr;52(3):343-51. doi: 10.1111/j.1574-695X.2008.00381.x. Epub 2008 Mar 6.
The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free.
描述了从一名7个月大儿童粪便样本中分离出的K/2002毒株的遗传特性，该儿童在3个月大时接种了第一剂口服脊髓灰质炎疫苗（OPV）。对分离株K/2002的初步测序表征显示，在VP1编码区的3'端发生了S3/S2重组事件。重组事件导致在Sabin 3基因组背景中引入了6个Sabin 2氨基酸残基。此外，在分离株K/2002的基因组中已鉴定出与Sabin 3毒株减毒表型丧失相关的突变。此处提供的数据强调了精心规划疫苗接种策略的必要性，其中包括在已认证无脊髓灰质炎的地区停止使用OPV。
