[多聚腺苷二磷酸核糖聚合酶对失血性休克大鼠血管低反应性的影响]

[Effect of poly-adenosine diphosphate ribosyl-polymerase on vascular hyporeactivity in rats with hemorrhagic shock].

作者信息

Zhou Xue-wu, Liu Jian-cang, Liao Zi-fu, Wang Li-ting, Liu Liang-ming

机构信息

State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing, China.

出版信息

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2008 Mar;20(3):148-50.

PMID:18328126

Abstract

OBJECTIVE

To study the effects of poly-adenosine diphosphate ribosyl-polymerase (PARP) on vascular hyporeactivity during hemorrhagic shock in rats.

METHODS

Sprague-Dawley (SD) rats were randomly divided into three groups: shock, 3-aminobenzamide (3-AB) pretreatment+shock, and sham operation. Bleeding from the femoral artery to induce hemorrhagic shock model. The blood pressure changes following 3 microg/kg norepinephrine (NE) injection were observed in vivo. The response of vascular rings of superior mesenteric artery (SMA) to NE was determined ex vivo. The nitrogen monoxide (NO) contents of plasma and tissue homogenate of SMA were measured using the assay kit based on the nitrate reductase reaction.

RESULTS

The maximum increase of mean arterial pressure in response to NE immediately following shock in the shock group was significantly lower than in the sham operation group (P<0.01) and the value at 1 hour after blood reinfusion in the shock group was obviously lower than in the 3-AB pretreatment+shock group (P<0.05) and in the sham operation group (P<0.01). The maximum concentration force in the sham operation group [(0.367 1+/-0.221 3)g/mm] was significantly increased than in the 3-AB pretreatment+shock group [(0.286 4+/-0.153 2) g/mm, P<0.05] and in the shock group [(0.185 6+/-0.111 3)g/mm, P<0.01]. The cumulative dose-response curves of SMA to NE shifted to the left, and the contraction force was markedly increased as NE concentration reaching 10(-6), 10(2+) and 10(-5) mol/L in the 3-AB pretreatment+shock group compared to the shock group (all P<0.05). There were no significant difference on plasma NO content among the three groups. However, the NO contents of plasma and tissue homogenate of SMA in the 3-AB pretreatment+ shock group were slightly lower than in the shock group (P>0.05).

CONCLUSION

PARP is involved in the vascular hyporeactivity in hemorrhagic-shocked rats.

摘要

目的

研究聚腺苷二磷酸核糖聚合酶（PARP）对大鼠失血性休克期间血管低反应性的影响。

方法

将Sprague-Dawley（SD）大鼠随机分为三组：休克组、3-氨基苯甲酰胺（3-AB）预处理+休克组和假手术组。通过股动脉放血诱导失血性休克模型。在体内观察注射3μg/kg去甲肾上腺素（NE）后血压的变化。离体测定肠系膜上动脉（SMA）血管环对NE的反应。使用基于硝酸还原酶反应的试剂盒测定SMA血浆和组织匀浆中的一氧化氮（NO）含量。

结果

休克组休克后立即给予NE时平均动脉压的最大升高幅度显著低于假手术组（P<0.01），休克组回输血液1小时后的数值明显低于3-AB预处理+休克组（P<0.05）和假手术组（P<0.01）。假手术组的最大收缩力[(0.367 1±0.221 3)g/mm]显著高于3-AB预处理+休克组[(0.286 4±0.153 2) g/mm，P<0.05]和休克组[(0.185 6±0.111 3)g/mm，P<0.01]。与休克组相比，3-AB预处理+休克组中SMA对NE的累积剂量-反应曲线左移，当NE浓度达到10(-6)、10(2+)和10(-5) mol/L时收缩力明显增加（均P<0.05）。三组血浆NO含量差异无统计学意义。然而，3-AB预处理+休克组SMA血浆和组织匀浆中的NO含量略低于休克组（P>0.05）。