[抗原特异性Th17细胞的分化与调控]

[The differentiation and regulation of antigen-specific Th17 cells].

作者信息

Yang Jing, Jia Lei, Li Li, Wu Chang-you

机构信息

Department of Immunology, State Ministry of Education Key Laboratory of Tropical Disease Control, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Mar;24(3):213-6.

PMID:18328176

Abstract

AIM

To assess the differentiation and regulation of antigen specific Th17 cells in vitro.

METHODS

Naive CD4(+) T cells from OVA-TCR-transgenic mice (OVA-TCR-Tg) were isolated and co-cultured with splenocytes from normal BALB/c mice under different culture conditions. The expression and production of IL-17 and other cytokines were assessed.

RESULTS

OVA peptide alone mainly induced Th1 type responses. Addition of TGF-beta plus IL-6 induced Th17 response. Furthermore, addition of IL-23 to cell culture could promote the differentiation of Th17 cells. Blocking of IFN-gamma and IL-4 by neutralizing monoclonal antibodies enhanced the percentage of IL-17-producing cells. LPS could promote the production of Th1 cytokines but had no significant effect on IL-17 expression.

CONCLUSION

Antigen specific Th17 cells are distinct from antigen specific Th1 and Th2 cells. TGF-beta, IL-6 and IL-23 are the factors responsible for promoting the differentiation and development of Th17 subset, whereas IFN-gamma and IL-4 have inhibitory effects.

摘要

目的

在体外评估抗原特异性Th17细胞的分化及调控。

方法

从OVA-TCR转基因小鼠（OVA-TCR-Tg）中分离出初始CD4(+) T细胞，并在不同培养条件下与正常BALB/c小鼠的脾细胞共培养。评估白细胞介素-17（IL-17）及其他细胞因子的表达和产生情况。

结果

单独的OVA肽主要诱导Th1型反应。添加转化生长因子-β（TGF-β）加白细胞介素-6（IL-6）诱导Th17反应。此外，向细胞培养物中添加白细胞介素-23（IL-23）可促进Th17细胞的分化。通过中和单克隆抗体阻断干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）可提高产生IL-17细胞的百分比。脂多糖（LPS）可促进Th1细胞因子的产生，但对IL-17表达无显著影响。