Bugyi Beáta, Le Clainche Christophe, Romet-Lemonne Guillaume, Carlier Marie-France
Cytoskeleton Dynamics and Motility Group, LEBS, UPR 3082, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
FEBS Lett. 2008 Jun 18;582(14):2086-92. doi: 10.1016/j.febslet.2008.02.065. Epub 2008 Mar 5.
Recent live cell image analysis of actin dynamics in lamellipodia of motile cells has shown that regulated treadmilling, which supports actin-based propulsion of functionalized particles in biomimetic reconstituted motility assays, is also responsible for lamellipodia extension. In both cases, filaments are created by branching with Arp2/3 complex only at the membrane or particle surface, grow transiently and are capped; ADF/cofilin enhances the treadmilling but does not sever filaments in the body of the meshwork. Differences between the cellular and biomimetic systems suggest that additional regulatory mechanisms take place in lamellipodia.
最近对运动细胞片状伪足中肌动蛋白动力学的活细胞图像分析表明，在仿生重组运动测定中支持基于肌动蛋白的功能化颗粒推进的调节性肌动蛋白踏车行为，也是片状伪足延伸的原因。在这两种情况下，细丝仅在膜或颗粒表面通过与Arp2/3复合物分支产生，短暂生长并被加帽；ADF/丝切蛋白增强肌动蛋白踏车行为，但不会切断网络主体中的细丝。细胞系统和仿生系统之间的差异表明，片状伪足中存在额外的调节机制。
